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Protein phosphatase 1 suppresses androgen receptor ubiquitylation and degradation.
Liu X, Han W, Gulla S, Simon NI, Gao Y, Cai C, Yang H, Zhang X, Liu J, Balk SP, Chen S
Oncotarget. 2016 Jan 12;7(2):1754-64. doi: 10.18632/oncotarget.6434.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
171234HA-HA-BI-ARMammalian expression of 2xHA-tagged human androgen receptor (hAR)
171235HA-HA-AR-NTDMammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)
171236HA-HA-AR-NTD-DBDMammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain and DNA-bindind domain (NTD-DBD)
171237HA-HA-AR-DBD-LBDMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)
171238HA-HA-AR-DBDMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)
171239HA-AR-LBDMammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)
171245Flag-M4-AR-S650AMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Ala (AR-S650A)
171246Flag-M4-AR-S650DMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Asp (AR-S650D)
171252Flag-M4-AR-W741C-S650AMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Ala (AR-W741C-S650A)
171253Flag-M4-AR-W741C-S650DMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser650Asp (AR-W741C-S650D)
171267Flag-neo-PP1-AlphaMammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit
171268Flag-neo-PP1-Alpha-H248KMammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant that loses enzymatic activity

Antibodies from Article