Allosteric regulation within the highly interconnected structural scaffold of AraC/XylS homologs tolerates a wide range of amino acid changes.

Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, Swint-Kruse L
Proteins. 2021 Aug 8. doi: 10.1002/prot.26206. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
170107	UUUUA-pHG165c	Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the UreR urea ligand binding domain.
170108	MMMMA-pHG165c	Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the MelR L-melibiose ligand binding domain.
170110	XXXXA-pHG165c	Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.
170111	XXXXA_Q182A_R287H-pHG165c	Constitutively expressed , mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.
170112	RRRAA-pHG165c	Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.
170113	RRRAA-5-mut-pHG165c	Constitutively expressed, mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.
170114	AraC-pHG165c WT	Constitutively expressed, wild-type E coli AraC transcription activator; activated by L-Arabinose.
172602	BW-Para	The BW-Para E. coli strain is used to screen the functions of chimeric AraC/XylS transcription activators using beta-galactosidase assays.