Rapid characterization of spike variants via mammalian cell surface display.
Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 10.1016/j.molcel.2021.11.024.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172721 | Spike Display_Part 1 DO | Encodes a sfGFP dropout expression cassette in place of Part 1 of HexaPro-D614G spike to be used in the Spike Display system |
| 172722 | Spike Display_Part 2 DO | Encodes a sfGFP dropout expression cassette in place of Part 2 of HexaPro-D614G spike to be used in the Spike Display system |
| 172723 | Spike Display_Part 3 DO | Encodes a sfGFP dropout expression cassette in place of Part 3 of HexaPro-D614G spike to be used in the Spike Display system |
| 172724 | Spike Display_Part-4 DO | Encodes a sfGFP dropout expression cassette in place of Part 4 of HexaPro-D614G spike to be used in the Spike Display system |
| 172725 | Spike Display_Part-5 DO | Encodes a sfGFP dropout expression cassette in place of Part 5 of HexaPro-D614G spike to be used in the Spike Display system |
| 172726 | Spike Display_Part 1-5 DO | Encodes a sfGFP dropout expression cassette in place of Parts 1-5 to be used in the Spike Display system |
| 172727 | Spike Display_Part 1a Spacer | Encodes Part 1a of HexaPro-D614G spike to be used in the Spike Display system |
| 172728 | Spike Display_Part 1b Spacer | Encodes Part 1b of HexaPro-D614G spike to be used in the Spike Display system |
| 172729 | Spike Display_Part 1 Spacer | Encodes Part 1 of HexaPro-D614G spike to be used in the Spike Display system |
| 172730 | Spike Display_Part 2 Spacer | Encodes Part 2 of HexaPro-D614G spike to be used in the Spike Display system |
| 172731 | Spike Display_Part 3 Spacer | Encodes Part 3 of HexaPro-D614G spike to be used in the Spike Display system |
| 172732 | Spike Display_Part 4 Spacer | Encodes Part 4 of HexaPro-D614G spike to be used in the Spike Display system |
| 172733 | Spike Display_Part 5 Spacer | Encodes Part 5 of HexaPro-D614G spike to be used in the Spike Display system |
| 172734 | Spike Display_6P-D614G | Expression vector for SARS-CoV-2 (HexaPro-D614G) spike used in the Spike Display system |
| 172735 | Spike Display_B.1.1.7 | Expression vector for SARS-CoV-2 (B.1.1.7) spike used in the Spike Display system |
| 172736 | Spike Display_B.1.351 | Expression vector for SARS-CoV-2 (B.1.351) spike used in the Spike Display system |
| 172737 | Spike Display_B.1.1.248 | Expression vector for SARS-CoV-2 (B.1.1.248) spike used in the Spike Display system |
| 172738 | Spike Display_B.1.427/B.1.429 | Expression vector for SARS-CoV-2 (B.1.427/B.1.429) spike used in the Spike Display system |