Transcriptional suppression of ribosomal DNA with phase separation.

Ide S, Imai R, Ochi H, Maeshima K
Sci Adv. 2020 Oct 14;6(42). pii: 6/42/eabb5953. doi: 10.1126/sciadv.abb5953. Print 2020 Oct. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
247346	pX330-TAF1-A	Expresses SpCas9 and a sgRNA targeting the human TAF1-A loci for knock-in.
247347	pEF1-FRT-HaloTag-TAF1A	Expresses HaloTag-TAF1A
247350	pX330-UBF	Expresses SpCas9 and a sgRNA targeting the human UBF loci for knock-in.
247351	pGEM-HaloTag-UBF	CRISPR/Cas9 donor plasmid to tag human UBF with Halo tag
247352	pX330-RPA194	Expresses SpCas9 and a sgRNA targeting the human RPA194 loci for knock-in.
247353	pGEM-HaloTag-RPA194	CRISPR/Cas9 donor plasmid to tag human RPA194 with Halo tag
247354	pGEM-PAmCherry-RPA194	CRISPR/Cas9 donor plasmid to tag human RPA194 with PAmCherry
247355	pGEM-mAID-RPA194	CRISPR/Cas9 donor plasmid to tag human RPA194 with mAID
247356	pX330-AAVS1	Expresses SpCas9 and a sgRNA targeting the human AAVS1 loci for knock-in.