The pIT5 Plasmid Series, an Improved Toolkit for Repeated Genome Integration in E. coli.
Hao N, Chen Q, Dodd IB, Shearwin KE
ACS Synth Biol. 2021 Jun 30. doi: 10.1021/acssynbio.1c00215.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173767 | pIT5_CL | Bacterial genomic integration vector |
| 173768 | pIT5_TL | Bacterial genomic integration vector |
| 173769 | pIT5_SL | Bacterial genomic integration vector |
| 173770 | pIT5_KL | Bacterial genomic integration vector |
| 173771 | pIT5_KD | Bacterial genomic integration vector |
| 173772 | pIT5_KH | Bacterial genomic integration vector |
| 173773 | pIT5_KT | Bacterial genomic integration vector |
| 173774 | pIT5_KP | Bacterial genomic integration vector |
| 173775 | pIT5_KO | Bacterial genomic integration vector |
| 173776 | pIT5_KW | Bacterial genomic integration vector |
| 173777 | pIT5_KW_CymR | Bacterial genomic integration vector, CymR repressor |
| 173778 | pIT5_KD_NahR | Bacterial genomic integration vector, NahR repressor |
| 173779 | pIT5_KH_VanR | Bacterial genomic integration vector, VanR repressor |
| 173780 | pIT5_KP_LacI | Bacterial genomic integration vector, LacI repressor |
| 173781 | pHelper_D | The D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid. |
| 173782 | pAH69 | The pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid. |
| 173783 | pHelper_O | The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid. |
| 173784 | pHelper_W | The Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid. |
| 173785 | pE_Cre_lambdaCos | The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants. |