The modular pYT vector series employed for chromosomal gene integration and expression to produce carbazoles and glycolipids in P. putida.
Weihmann R, Kubicki S, Bitzenhofer NL, Domrose A, Bator I, Kirschen LM, Kofler F, Funk A, Tiso T, Blank LM, Jaeger KE, Drepper T, Thies S, Loeschcke A
FEMS Microbes. 2022 Dec 19;4:xtac030. doi: 10.1093/femsmc/xtac030. eCollection 2023.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177278 | pYTRW10K_0x5 | genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 |
| 177279 | pYTRW07K_0G5 | genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR |
| 177280 | pYTRW08K_0C5 | genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with CmR |
| 177281 | pYTRW09K_0T5 | genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR |
| 177282 | pYTRW11K_0S5 | genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with SmR |
| 177283 | pYTRW14K_7G5 | genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and LacZ |
| 177284 | pYTRW15K_2G5 | genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and mTagBFP2 |
| 177285 | pYTRW17K_6G5 | genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and PE-H |
| 177286 | pYTRW16K_1G5 | genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and eYFP |
| 177287 | pYTRW13K_3G5 | genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and mCherry |
| 177288 | pYTRW18K_3T5 | genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR and mCherry |
| 177289 | pYTRW20K_0Ti1 | genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR |
| 177290 | pYTRW28K_0Ti1 | genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR |
| 177291 | pYTRW21K_1Ti1 | genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and eYFP |
| 177292 | pYTRW26K_1Ti1 | genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and eYFP |
| 177293 | pYTRW22K_7Ti1 | genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and LacZ |
| 177294 | pYTRW27K_7Ti1 | genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and LacZ |
| 177301 | pYTSK56K_3G7 | genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and mCherry |
| 177302 | pYTSK58K_6G7 | genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and PE-H |
| 177303 | pYTSK65K_8G7 | genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR and GUS |
| 177307 | yTREX-ApR | genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 |