Real-time detection of response regulator phosphorylation dynamics in live bacteria.
Butcher RJ, Tabor JJ
Proc Natl Acad Sci U S A. 2022 Aug 30;119(35):e2201204119. doi: 10.1073/pnas.2201204119. Epub 2022 Aug 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 181944 | pRB035 | aTc-inducible expression of mNeonGreen |
| 181945 | pRB121 | aTc-inducible expression of dimerized mNeonGreen (mNG-mNG) |
| 181946 | pRB116.2 | aTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129 |
| 181947 | pRB120.2 | aTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129 |
| 181948 | pRB113.2 | aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS |
| 181949 | pRB117.2 | aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS |
| 181950 | pRB133.2 | aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK |
| 181951 | pKD317.8 | IPTG-inducible expression of NarX |
| 181952 | pKB083 | IPTG-inducible expression of PhoQ |