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Addgene
Relaxation Sensors
Seidel ZP, Wang JCK, Riegler J, York AG, Ingaramo M
(2021), doi: 10.5281/zenodo.5810930 Article

Plasmids from Article

ID Plasmid Purpose
182299pQE31-6xHis-CountdownE. coli plasmid encoding Countdown with a hexahistidine tag
182300pQE31-6xHis-pHCountdownE. coli plasmid encoding pH-Countdown with a hexahistidine tag
182301pCDH-EF1-pHCountdownlentiviral plasmid encoding pH-Countdown and puromycin selection
182302pCDH-EF1-Mito-pHCountdownlentiviral plasmid encoding pH-Countdown targeted to mitochondria via fusion to the COX8A presequence with puromycin selection
182303N1-Mito-pHCountdownmammalian cell plasmid encoding pH-Countdown targeted to mitochondria via fusion to the COX8A presequence
182304pUC57-Peft-pHCountdown-unc54C. elegans plasmid encoding pH-Countdown with three introns
182305pRSET-TorA-6xHis-M13-pHCountdown-CalmodulinE. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.

Antibodies from Article