Endoplasmic reticulum stress activates human IRE1alpha through reversible assembly of inactive dimers into small oligomers.
Belyy V, Zuazo-Gaztelu I, Alamban A, Ashkenazi A, Walter P
Elife. 2022 Jun 22;11:e74342. doi: 10.7554/eLife.74342.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 185676 | pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm | SpCas9 and gRNA targeting the C-terminus of HsIRE1 |
| 185677 | pPW3755 : HDR-HsIRE1a-10xGS-HaloTag | HDR template used for endogenous CRISPR editing of ERN1 gene, inserting a C-terminal HaloTag. |
| 185678 | pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP | Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane. |
| 185679 | pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP | Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane. |
| 185680 | pPW3758 : CMVd3-HsIRE1-HaloTag | Low-level transient expression of full-length HsIRE1 with a C-terminal HaloTag. |
| 185681 | pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag | Low-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag. |
| 185682 | pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag | Low-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag. |
| 185683 | pPW3761 : CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag | Low-level transient expression of IF2 mutant HsIRE1 with a C-terminal HaloTag. |
| 185684 | pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag | Low-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag. |
| 185685 | pPW3763 : CMVd3-HsIRE1dLKR-HaloTag | Low-level transient expression of HsIRE1 lacking the linker, kinase, and RNase domains, with a C-terminal HaloTag. |
| 185686 | pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP | Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization. |
| 185687 | pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP | Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer). |