Engineered serum markers for non-invasive monitoring of gene expression in the brain.
Lee S, Nouraein S, Kwon JJ, Huang Z, Wojick JA, Xia B, Corder G, Szablowski JO
Nat Biotechnol. 2024 Jan 10. doi: 10.1038/s41587-023-02087-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 189624 | pAAV-hSyn-Cluc-RMA | Expresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction. |
| 189627 | pAAV-hSyn-hM3D(Gq)::RAM-d2tTA | Expresses hM3D(Gq) under the hSyn promoter, and d2tTA under the pRAM (synthetic Fos) promoter. Plasmid 1 for recording Fos expression. |
| 189628 | pAAV-TRE-Gluc-RMA-IRES-EGFP | Expresses Gluc-RMA and EGFP driven by the Tet Response Element (TRE). Plasmid 2 for recording Fos expression. |
| 189629 | pAAV-hSyn-Gluc-RMA-IRES-EGFP | Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction. |
| 189630 | pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP | Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations. |