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Inducible directed evolution of complex phenotypes in bacteria.

Al'Abri IS, Haller DJ, Li Z, Crook N
Nucleic Acids Res. 2022 Jun 10;50(10):e58. doi: 10.1093/nar/gkac094. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
189935ISA431: aTc-MP6Mutagenesis Plasmid: pTet, danQ926, dam, seqA, emrR, ugi and cda1, tetR, CloDF13, KanR
241968ISA179: Tagatose pathwayPhagemid for the tagatose pathway. Contains p15A, CmR, pac site, AraC, C1, and pBAD.
241969ISA410: sfGFPPhagemid for SC101 and sfGFP. Contains p15A, CmR, pac site, AraC, C1, and pBAD.
241971ISA861: Melezitose pathwayMelezitose pathway phagemid. Contains p15A, CmR, pac site, AraC, C1, and pBAD.
241972ISA894: vioABCDE phagemidcoi(Y35H) phagemid with higher infection rate (unpublished)

Antibodies from Article