Endogenous transcripts direct microRNA degradation in Drosophila, and this targeted degradation is required for proper embryonic development.
Kingston ER, Blodgett LW, Bartel DP
Mol Cell. 2022 Sep 15. pii: S1097-2765(22)00849-8. doi: 10.1016/j.molcel.2022.08.029.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190608 | HA-doraWT_rescue_construct | Puromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cells |
| 190609 | doraWT_rescue_construct | Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells |
| 190637 | dora[B]_rescue_construct | Puromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells |
| 190638 | HA-dora[B]_rescue_construct | Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells |
| 190639 | dora[A]_rescue_construct | Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells |
| 190640 | HA-dora[A]_rescue_construct | Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells |
| 190641 | HA-GFP_rescue_construct | Puromycin-selectable expression of GFP in Drosophila S2 cells |
| 190700 | pAc-sgRNA-Cas9.dora_1 | Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells |
| 190701 | pAc-sgRNA-Cas9.dora_2 | Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells |
| 190702 | pAc-sgRNA-Cas9.dora_3 | Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells |
| 190703 | pAc-sgRNA-Cas9.scr_1 | Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells |
| 190704 | pAc-sgRNA-Cas9.scr_2 | Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells |
| 190705 | pAc-sgRNA-Cas9.scr_3 | Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells |