Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids.
Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M
Nat Commun. 2025 Jul 1;16(1):5784. doi: 10.1038/s41467-025-60949-1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191211 | pLEX-307_LgBiT | Lentiviral-mediated stable expression of LgBiT for slit nanoluciferase complementation assays in mammalian cells |
| 191212 | pcDNA3.1-SARS2-Spike-HiBiT | Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay |
| 191214 | pLD-puro-Cc-SARS-CoV-2_NSP3-VA | Lentiviral expression of SARS-CoV-2 NSP3 in mammalian cells |
| 191215 | pLD-puro-Cc-SARS-CoV-2_NSP16-VA | Lentiviral expression of SARS-CoV-2 NSP16 in mammalian cells |
| 191216 | pLD-puro-Cc-SARS-CoV-2_Spike_WT-VA | Lentiviral expression of SARS-CoV-2 Spike_WT in mammalian cells |
| 191217 | pLD-puro-Cc-SARS-CoV-2_Spike_Alpha-VA | Lentiviral expression of SARS-CoV-2 Spike_Alpha in mammalian cells |
| 191218 | pLD-puro-Cc-SARS-CoV-2_Spike_Beta-VA | Lentiviral expression of SARS-CoV-2 Spike_Beta in mammalian cells |
| 191219 | pLD-puro-Cc-SARS-CoV-2_Spike_Delta-VA | Lentiviral expression of SARS-CoV-2 Spike_Delta in mammalian cells |
| 191220 | pLD-puro-Cc-SARS-CoV-2_Spike_Lambda-VA | Lentiviral expression of SARS-CoV-2 Spike_Lambda in mammalian cells |
| 191221 | pLD-puro-Cc-IFIT5_WT-VA | Lentiviral expression of human IFIT5_WT in mammalian cells |
| 191222 | pLD-puro-Cc-IFIT5_K160R-VA | Lentiviral expression of human IFIT5_K160R in mammalian cells |
| 191223 | pLD-puro-Cc-IFIT5_K197R-VA | Lentiviral expression of human IFIT5_K197R in mammalian cells |
| 191224 | pLD-puro-Cc-IFIT5_K206R-VA | Lentiviral expression of human IFIT5_K206R in mammalian cells |
| 214429 | pLEX_307-SARS-CoV-2-NSP3-V5 | Lentiviral-mediated stable expression of SARS-CoV-2 NSP3 in mammalian cells |