A continuous epistasis model for predicting growth rate given combinatorial variation in gene expression and environment.
Otto RM, Turska-Nowak A, Brown PM, Reynolds KA
Cell Syst. 2024 Feb 21;15(2):134-148.e7. doi: 10.1016/j.cels.2024.01.003. Epub 2024 Feb 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191856 | pCRISPR3_BC1 | Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region. |
| 191857 | pCRISPR3_BC2 | Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region. |
| 191858 | pCRISPR3_BC3 | Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region. |
| 191859 | pCRISPR3_BC4 | Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region. |
| 191860 | pCRISPR3_BC5 | Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region. |
| 191861 | pCRISPR3_BC6 | Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region. |