Expanding the Scope of Bacterial CRISPR Activation with PAM-Flexible dCas9 Variants.
Kiattisewee C, Karanjia AV, Legut M, Daniloski Z, Koplik SE, Nelson J, Kleinstiver BP, Sanjana NE, Carothers JM, Zalatan JG
ACS Synth Biol. 2022 Nov 15. doi: 10.1021/acssynbio.2c00405. PubMed Article
Kiattisewee C, Karanjia AV, Legut M, Daniloski Z, Koplik SE, Nelson J, Kleinstiver BP, Sanjana NE, Carothers JM, Zalatan JG
ACS Synth Biol. 2022 Nov 15. doi: 10.1021/acssynbio.2c00405. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192637 | pCK668 | Expresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR |
| 192638 | pCK669 | Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR |
| 192639 | pCK340 | Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR |
| 192640 | pCK341 | Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR |
| 192641 | pCK671.306 | Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR |
| 192642 | pCK364.306 | Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR |
| 192643 | pCK760 | Expresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpR |
| 192644 | pCK411.RR1 | Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR |