Single-molecule imaging reveals translation-dependent destabilization of mRNAs.
Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA
Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196918 | SL-TREAT | The TREAT reporter containing cap-proximal GC-rich stemloop |
| 196919 | SL-SunTag | The SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich stem-loop |
| 196920 | IRE-SunTag | The 5UTR of the ferritin heavy chain mRNA was cloned upstream of the SunTag-Renilla coding sequence in the ΔIRE-SunTag reporter |
| 196921 | IRE-TREAT | The 5UTR of the ferritin heavy chain mRNA was cloned upstream of the Renilla coding sequence in the ΔIRE-TREAT reporter |
| 196922 | IRE-nonOpt-TREAT | The codon optimized Renilla coding sequence in the IRE-TREAT reporter was replaced by non-optimal Renilla containing 93 synonymous codon substitutions. |
| 196923 | IRE-TPI-wildtype-TREAT | The coding sequence of Triose phosphate isomerase gene was fused downstream Renilla in the ORF |
| 196925 | Let7-IRE-TREAT | Let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter. |
| 196926 | IRE-let7-SunTag-TREAT | SunTag cassette was added in the coding region in the let7-IRE-TREAT reporter |
| 196927 | IRE-let7R-SunTag-TREAT | SunTag cassette was added in the coding region in the let7R-IRE-TREAT reporter. |
| 196928 | Let7-SunTag | Let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter. |
| 196929 | Let7R-SunTag | Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter. |
| 196930 | miR21-IRE-TREAT | mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter. |
| 196931 | miR21mutant-IRE-TREAT | Mutated mir21 binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT reporter |
| 196932 | IRE-miR21-SunTag-TREAT | SunTag cassette was added in the coding region in the miR21-IRE-TREAT reporter. |
| 196933 | IRE-miR21mutant-SunTag-TREAT | SunTag cassette was added in the coding region in the miR21mutant -IRE-TREAT reporter. |
| 196934 | Let7R-IRE-TREAT | Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT. |