An engineered biosensor enables dynamic aspartate measurements in living cells.
Davidsen K, Marvin JS, Aggarwal A, Brown TA, Sullivan LB
eLife. 2024 Feb 23;12:RP90024. doi: 10.7554/eLife.90024.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203458 | pRSET-jAspSnFR3 | jAspSnFR3 in T7 bacterial expression vector |
| 203459 | pAAV-CAG-jAspSnFR3-mRuby3 | jAspSnFR3-mRuby3 in adenoviral expression vector |
| 203460 | pENTR1a-jAspSnFR3 | jAspSnFR3 in Gateway cloning entry vector |
| 203461 | pENTR1a-MTS-jAspSnFR3 | Mitochondrial directed jAspSnFR3 in Gateway cloning entry vector |
| 203487 | pLenti-MTS-jAspSnFR3 | Mitochondrial directed jAspSnFR3 in lentiviral expression vector |
| 203488 | pLenti-jAspSnFR3 | jAspSnFR3 in lentiviral expression vector |
| 203489 | pENTR1a-jAspSnFR3-mRuby3-NLS | Nuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vector |
| 203490 | pENTR1a-MTS-jAspSnFR3-mRuby3 | Mitochondrial directed jAspSnFR3-mRuby3 in Gateway cloning entry vector |
| 203491 | pENTR1a-jAspSnFR3-mRuby3 | jAspSnFR3-mRuby3 in Gateway cloning entry vector |
| 203492 | pLenti-MTS-jAspSnFR3-mRuby3 | Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector |
| 203494 | pLenti-jAspSnFR3-mRuby3-NLS | Nuclear directed jAspSnFR3-mRuby3 in lentiviral expression vector |
| 203498 | pLenti-jAspSnFR3-mRuby3 | jAspSnFR3-mRuby3 in lentiviral expression vector |
| 203500 | pLX304-CAG-jAspSnFR3-mRuby3 | jAspSnFR3-mRuby3 in lentiviral expression vector |