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Phosphorylation of VAMP3 couples IL-6 Exocytosis to dendritic cell activation.

Chen T, Psoma A, Mahajan S, Ter Beest M, Linders P, Franciosa G, Bianchi F, Bogaart GVD, Warner H
J Cell Sci. 2025 Sep 22:jcs.264139. doi: 10.1242/jcs.264139. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
193635VAMP3(S48A)-mCherryExpresses S48A phosphodead VAMP3 fused to mCherry
193636WDFY2-GFPExpresses GFP-tagged WDFY2 in mammalian cells
193637VAMP3(S48E)-mCherryExpresses S48E phosphomimetic VAMP3 fused to mCherry
206028hVAMP3(1-77) WTExpresses the SNARE motif of wildtype human VAMP3 in bacteria
206029hVAMP3(1-77) S44AExpresses the SNARE motif of human VAMP3 mutant S44A in bacteria
206030hVAMP3(1-77) S44EExpresses the SNARE motif of human VAMP3 mutant S44E in bacteria
206031hSTX4(1-271,272C)Bacterial expression of human Syntaxin 4 without transmembrane helix and a C-terminal cysteine

Antibodies from Article