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Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells.

Mikhova M, Goff NJ, Janovic T, Heyza JR, Meek K, Schmidt JC
Nat Commun. 2024 Nov 23;15(1):10159. doi: 10.1038/s41467-024-54545-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
207541N-Terminal Halo XRCC4Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.
207546N-terminal Halo-XLFHomologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.
207547N-terminal Halo-Ku70Homologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus.
207583HaloKu70 sgRNAsgRNA for the insert of the HaloTag at the endogenous loci of Ku70.
207605Halo XLF sgRNAsgRNA for the insert of the HaloTag at the endogenous loci of XLF
207606HaloXRCC4 sgRNAsgRNA for the insert of the HaloTag at the endogenous loci of XRCC4.

Antibodies from Article