Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells.
Mikhova M, Goff NJ, Janovic T, Heyza JR, Meek K, Schmidt JC
Nat Commun. 2024 Nov 23;15(1):10159. doi: 10.1038/s41467-024-54545-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207541 | N-Terminal Halo XRCC4 | Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus. |
| 207546 | N-terminal Halo-XLF | Homologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus. |
| 207547 | N-terminal Halo-Ku70 | Homologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus. |
| 207583 | HaloKu70 sgRNA | sgRNA for the insert of the HaloTag at the endogenous loci of Ku70. |
| 207605 | Halo XLF sgRNA | sgRNA for the insert of the HaloTag at the endogenous loci of XLF |
| 207606 | HaloXRCC4 sgRNA | sgRNA for the insert of the HaloTag at the endogenous loci of XRCC4. |
| 210787 | Halo-DNAPKcs | Plasmid for expression of HaloTag-DNA-PKcs in mammalian cells |