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Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants.

Koeppel J, Weller J, Peets EM, Pallaseni A, Kuzmin I, Raudvere U, Peterson H, Liberante FG, Parts L
Nat Biotechnol. 2023 Feb 16. doi: 10.1038/s41587-023-01678-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
196962pCMV-PE2-P2A-PuroRExpresses PE2 with puromycin resistance
196970pLentiGuide-BlastRLentiviral acceptor vector for pegRNAs with blasticidin resistance
196971pPB-TREG3G-PE2-rtTA3G-P2A-eGFPPiggybac vector with doxycyclin-inducible prime editor for mammalian cells

Antibodies from Article