Imaging of genomic loci in Trypanosoma brucei using an optimised LacO-LacI system.
Budzak J, Goodwin I, Tiengwe C, Rudenko G
Mol Biochem Parasitol. 2023 Dec;256:111598. doi: 10.1016/j.molbiopara.2023.111598. Epub 2023 Nov 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210213 | p177bp-LacOx50-Hyg | Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region. |
| 210215 | p177bp-LacOx162-Hyg | Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region. |
| 210219 | prDNA spacer-LacOx50-20xMS2-Hyg | Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer. |
| 210220 | prDNA spacer-LacOx120-20xMS2-Hyg | Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer. |
| 210221 | pBES1-LacOx50-blast | Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter. |
| 210224 | pSMOX-mNG::LacI-V3 | Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus. |
| 210260 | pBES1-LacOx50-mCh-bla | Integrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter. |
| 210261 | pBES1-LacOx50-Hyg | Integrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter. |