Kevin Weeks Lab: Plasmids for measuring proximity-mediated function of RNA regulatory proteins
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 209936 | pA-CBh-TP-(inactive)CNOT7-V5H6.MCh.Puro | In mammalian cells expresses TP fused to an inactive CNOT7 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209937 | pA-CBh-TP-(inactive)CNOT7(minusCNOT6interaction)-V5H6.MCh.Puro | In mammalian cells expresses TP fused to an inactive CNOT7 that does not interact with CNOT6 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209938 | pA-CBh-TP-(inactive)CNOT7(minusCNOT6NOT1interaction)-V5H6.MCh.Puro | In mammalian cells expresses TP fused to an inactive CNOT7 that does not interact with CNOT6 or NOT1 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209939 | pA-CBh-TP-(inactive)CNOT7(minusNOT1interaction)-V5H6.MCh.Puro | In mammalian cells expresses TP fused to an inactive CNOT7 that does not interact with NOT1 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209940 | pA-CBh-TP-CNOT7(minusCNOT6interaction)-V5H6.MCh.Puro | In mammalian cells expresses TP fused to a CNOT7 that does not interact with CNOT6 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209941 | pA-CBh-TP-CNOT7(minusCNOT6NOT1interaction)-V5H6.MCh.Puro | In mammalian cells expresses TP fused to a CNOT7 that does not interact with CNOT6 or NOT1 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209942 | pA-CBh-TP-CNOT7(minusNOT1interaction)-V5H6.MCh.Puro | In mammalian cells expresses TP fused to a CNOT7 that does not interact with NOT1 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209943 | pA-CBh.TP-(inactive)CNOT6-V5H6.MCh.Puro | In mammalian cells expresses TP fused to an inactive CNOT6 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209944 | pA-CBh.TP-CNOT6-V5H6.MCh.Puro | In mammalian cells expresses TP fused to a CNOT6 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209945 | pA-CBh.TP-CNOT6L-V5H6.MCh.Puro | In mammalian cells expresses TP fused to a CNOT6L with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209946 | pA-CBh.TP-CNOT7-V5H6.MCh.Puro | In mammalian cells expresses TP fused to a CNOT7 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209947 | pA-CBh.TP-CNOT8-V5H6.MCh.Puro | In mammalian cells expresses TP fused to a CNOT8 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209948 | pA-CBh.TP-DCP2-V5H6.MCh.Puro | In mammalian cells expresses TP fused to a DCP2 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209949 | pA-CBh.TP(N55D)-CNOT7-V5H6.MCh.Puro | In mammalian cells expresses non-binding TP mutant fused to a CNOT7 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209950 | pA.CBh.TP-V5H6.MCh.Puro | In mammalian cells expresses TP with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209951 | pA.CBh.TP-ZFP36L2-V5H6.MCh.Puro | In mammalian cells expresses TP fused to a ZFP36L2 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker. |
| 209952 | pNL3.2.CMV.3UTR-TH.NLuc-PEST.Hygro | In mammalian cells expresses NanoLuc mRNA with the TH in the 3'-UTR. Also expresses a hygromycin resistance marker. |
| 209953 | pNL3.2.CMV.5UTR-TH.NLuc-PEST.Hygro | In mammalian cells expresses NanoLuc mRNA with the TH in the 5'-UTR. Also expresses a hygromycin resistance marker. |
| 209954 | pNL3.2.CMV.CDS1-TH.NLuc-PEST.Hygro | In mammalian cells expresses NanoLuc mRNA with the TH in the CDS1 position. Also expresses a hygromycin resistance marker. |
| 209955 | pNL3.2.CMV.CDS2-TH.NLuc-PEST.Hygro | In mammalian cells expresses NanoLuc mRNA with the TH in the CDS2 position. Also expresses a hygromycin resistance marker. |
| 209998 | pNL3.2.CMV.NLuc-PEST.Hygro | In mammalian cells expresses NanoLuc mRNA with no TH as a control. Also expresses a hygromycin resistance marker. |