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Visible Light-Induced Specific Protein Reaction Delineates Early Stages of Cell Adhesion.
Rahikainen R, Vester SK, Turkki P, Janosko CP, Deiters A, Hytonen VP, Howarth M
J Am Chem Soc. 2023 Nov 15;145(45):24459-24465. doi: 10.1021/jacs.3c07827. Epub 2023 Oct 31. PubMed Article

Plasmids from Article

ID Plasmid Purpose
210021TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTagExpression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface.
210022SpyCatcher003(K31TAG)-Talin rod-mScarletIExpression of SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI in mammalian cells
210023EGFP-Talin head-SpyCatcher003(K31TAG)Expression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cells
210024LifeAct-mNeonGreen -IRES- Talin head-SpyTag003Expression of LifeAct-mNeonGreen and Talin head(1-433)-SpyTag003 in mammalian cells
210025SpyTag003(M115A)-Talin rod-mCherryExpression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cells
210026SpyTag003(M115G)-Talin rod-mCherryExpression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in mammalian cells
210027SpyTag003(V114T, V116T)-Talin rod-mCherryExpression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cells

Antibodies from Article