Structure and activation of the human autophagy-initiating ULK1C:PI3KC3-C1 supercomplex
Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
bioRxiv 2023.06.01.543278
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203542 | pCAG-FIP200 (1-640) | Make FIP200 NTD:ATG13:ULK1 complex |
| 203543 | pCAG-FIP200(1-640)-MBP | FIP200 NTD-GFP mammalian expression |
| 203544 | pCAG-FIP200 (1-640)-GST | FIP200 NTD-MBP mammalian expression |
| 203545 | pCAG-FIP200 (1-640)-TSF | FIP200 NTD-TwinStrep-Flag mammalian expression |
| 203546 | pCAG-FIP200 (1-640)-msfGFP | FIP200 NTD-GFP-10His mammalian expression |
| 203547 | pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050) | Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex |
| 203548 | pCAG-TSF-ATG13 (450-517)-ULK1 (836-1050) | Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex |
| 203549 | pCAG-ATG13 (363-517)-ULK1 (836-1050)-TSF | Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex |
| 203550 | pCAG-TSF-ATG13 (363-517) | Mammalian expression of TwinStrep-Flag tagged ATG13 (363-517) |
| 203551 | pCAG-GST-ATG13 (363-517) | Mammalian expression of GST tagged ATG13 (363-517) |
| 203552 | pCAG-GST-ATG13 (460-517) | Mammalian expression of GST tagged ATG13 (460-517) |
| 203553 | pCAG-GST-ATG13 (363-460) | Mammalian expression of GST tagged ATG13 (363-460) |
| 203554 | pCAG-GST-ATG13 (363-398) | Mammalian expression of GST tagged ATG13 (363-398) |
| 203555 | pCAG-GST-ATG13 (363-392) | Mammalian expression of GST tagged ATG13 (363-392) |
| 203556 | pCAG-GST-ATG13 (402-517) | Mammalian expression of GST tagged ATG13 (402-517) |
| 203557 | pCAG-GST-ATG13 (402-450) | Mammalian expression of GST tagged ATG13 (402-450) |
| 203558 | pCAG-GST-ATG13 (450-517) | Mammalian expression of GST tagged ATG13 (450-517) |
| 203559 | pCAG-GST-ATG13 (6-196) | Mammalian expression of GST tagged ATG13 (6-196) |
| 203560 | pCAG-msfGFP-ATG13 (1-197) | Mammalian expression of GFP tagged ATG13 (1-197) |
| 203561 | pCAG-ATG13 (363-517)-MBP | Mammalian expression of ATG13 (363-517) with MBP tag |
| 203562 | pCAG-ULK1 (836-1050)-MBP | Mammalian expression of ULK1 (836-1050) with MBP tag |
| 203563 | pCAG-VPS15-TSF | Mammalian expression of VPS15 with TwinStrep-Flag tag |
| 203564 | pCAG-VPS34 | Mammalian expression of VPS34 |
| 203565 | pCAG-ATG14 | Mammalian expression of ATG14 |
| 203566 | pCAG-GST-ATG14 | Mammalian expression of GST tagged ATG14 |
| 203567 | pCAG-mCherry-ATG14 | Mammalian expression of mCherry tagged ATG14 |
| 203568 | pCAG-BECN1 | Mammalian expression of BECN1 |
| 210839 | pCAG-FIP200 (1-640)-V314D-TSF | Mammalian expression of FIP200 NTD mutant |
| 210840 | pCAG-FIP200 (1-640)-R326D-TSF | Mammalian expression of FIP200 NTD mutant |
| 210841 | pCAG-FIP200 (1-640)-R334D-TSF | Mammalian expression of FIP200 NTD mutant |
| 210842 | pCAG-FIP200 (1-640)-L324D/R543D/R548D-TSF | Mammalian expression of FIP200 NTD mutant |
| 210843 | pCAG-FIP200 (1-640)-V44D-TSF | Mammalian expression of FIP200 NTD mutant |
| 210844 | pCAG-GST-ATG13 (460-517)-F466D/F470D | Mammalian expression of ATG13 (460-517) mutant |
| 210845 | pCAG-GST-ATG13 (460-517)-L476/L479D | Mammalian expression of ATG13 (460-517) mutant |
| 210846 | pCAG-GST-ATG13 (460-517)-L495/L499D | Mammalian expression of ATG13 (460-517) mutant |
| 210847 | pCAG-GST-ATG13 (460-517)-F509/F512D | Mammalian expression of ATG13 (460-517) mutant |
| 210848 | pCAG-GST-ATG13 (363-398)-F394D/M396D/F397D | Mammalian expression of ATG13 (363-398) mutant |
| 210849 | pCAG-GST-ATG13 (363-450) | Mammalian expression of ATG13 (363-450) mutant |
| 210850 | pCAG-GST-ATG13 (363-450)-F394D/F397D /E403K | Mammalian expression of ATG13 (363-450) mutant |
| 210851 | pCAG-GST-ATG13 (450-517)-A452D/F453D | Mammalian expression of ATG13 (450-517) mutant |
| 210852 | pCDH1-CMV-FIP200-mCherry-Hygro | Lentiviral expression vector encoding FIP200-mCherry |
| 210853 | pCDH1-CMV-FIP200-V44D-mCherry-Hygro | Lentiviral expression vector encoding FIP200 mutant-mCherry |
| 210854 | pCDH1-CMV-FIP200-V314D-mCherry-Hygro | Lentiviral expression vector encoding FIP200 mutant-mCherry |
| 210855 | pCDH1-CMV-FIP200-V44D/V314D-mCherry-Hygro | Lentiviral expression vector encoding FIP200 mutant-mCherry |
| 210856 | pCDH1-CMV-FIP200-L324D/R543D/R548D-mCherry-Hygro | Lentiviral expression vector encoding FIP200 mutant-mCherry |
| 210857 | pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro | Lentiviral expression vector encoding FIP200 mutant-mCherry |
| 210858 | pCAG-TSF-ATG101 | Mammalian expression of ATG101 |
| 222821 | pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)-msfGFP | Mammalian expression of TwinStrep-Flag -ATG13 C-terminal and ULK1 MIT domain fusion complex fused with GFP-10His |
| 222822 | His-GST-ATG13 (363-517)-ULK1 (836-1050) | Bacteria expression of His-GST -ATG13 C-terminal and ULK1 MIT domain fusion complex |
| 222823 | His-GST-ATG13 (363-517) | Bacteria expression of His-GST -ATG13 C-terminal |
| 222824 | MBP-ATG13 (363-517)-ULK1 (836-1050)-His | Bacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6His |
| 222825 | MBP-ATG13 (450-517)-ULK1 (836-1050)-His | Bacteria expression of MBP-ATG13 (450-517) and ULK1 MIT domain fusion complex -6His |