Saturation mutagenesis of a predicted ancestral Syk-family kinase.

Hobbs HT, Shah NH, Shoemaker SR, Amacher JF, Marqusee S, Kuriyan J
Protein Sci. 2022 Oct;31(10):e4411. doi: 10.1002/pro.4411. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
214235	pET23a-H6-TEV-AncSZ	Bacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylation