Front-biased activation of Ras-Rab5-Rac1 loop coordinates collective cell migration.
Jikko Y, Deguchi E, Matsuda K, Hino N, Tsukiji S, Matsuda M, Terai K
J Cell Sci. 2025 Jul 16:jcs.263779. doi: 10.1242/jcs.263779.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214818 | pPBbsr2-RaichuEV-Rac1HRasCT | Encoding a Förster resonance energy transfer (FRET) biosensor for Rac1 activity with cyan and yellow fluorescent protein. |
| 214819 | pCSIIbsr-Raichu-Rab5/PM | Encoding a Förster resonance energy transfer (FRET) biosensor for Rab5 activity with cyan and yellow fluorescent protein. |
| 214820 | pPBpuro-RasS17N | Encoding dominant negative HRas |
| 214821 | pPBpuro-Rac1T17N | Encoding dominant negative Rac1 |
| 214822 | pPBpuro-Rab5S34N | Encoding dominant negative Rab5A |
| 214823 | lentiCRISPRv2-Merlin(dog)gRNA1 | A knockout vector for dog Merlin. |
| 214824 | lentiCRISPRv2-bleo-Merlin(dog)gRNA2 | A knockout vector for dog Merlin. |
| 214827 | pPBpuro-miRFP703-eDHFR(69K6)-mSOS1-linkercat | Encoding mSOS1-linkercat fused to miRFP703 |
| 214828 | pPBpuro-chGrb2/eDHFR(69K6)-iRFP713 | Encoding Grb2-eDHFR(69K6) chimera fused to iRFP713 |
| 214829 | pPBpuro-ERK-KTR-mCherry | Encoding a fluorescent reporter for ERK activity |
| 214830 | pCSIIbleo-H2B-iRFP | Encoding nuclear-targeted iRFP713. |
| 228755 | pX459-SOS1(dog)-gRNA1 | A knockout vector for dog SOS1. |
| 228756 | pX459-SOS1(dog)-gRNA2 | A knockout vector for dog SOS1. |
| 228758 | pPBneo-UbC-mCherry-Merlin | Encoding Merlin fused to mCherry. |
| 228759 | pPBneo-UbC-mCherry | Encoding mCherry. |
| 228760 | pSUPER-Rac1(dog) | A knockdown vector for dog Rac1. |