Dynamic regulation of integrin beta1 phosphorylation supports invasion of breast cancer cells.
Conway JRW, Joshi O, Kaivola J, Follain G, Gounis M, Kuhl D, Ivaska J
Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215442 | pENTR221-Illusia | Gateway entry vector with Illusia FRET reporter for integrin phosphorylation |
| 215443 | pENTR221-Illusia(YYFF) | Gateway entry vector with inactive phospho-mutant (YYFF) of Illusia FRET reporter for integrin phosphorylation |
| 215444 | pPB.DEST-Illusia | Piggybac expression vector with Illusia FRET reporter for integrin phosphorylation |
| 215445 | pPB.DEST-Illusia(YYFF) | Piggybac expression vector with inactive phospho-mutant (YYFF) of Illusia FRET reporter for integrin phosphorylation |
| 215446 | pENTR2b-ITGB1(WT)-mRuby2 | Gateway entry vector with integrin beta1 tagged with mRuby2 |
| 215447 | pENTR2b-ITGB1(YYFF)-mRuby2 | Gateway entry vector with integrin beta1 NPxY mutant (YYFF) tagged with mRuby2 |
| 215448 | pPB.DEST-ITGB1(WT)-mRuby2 | Piggybac expression vector with integrin beta1 tagged with mRuby2 |
| 215449 | pPB.DEST-ITGB1(YYFF)-mRuby2 | Piggybac expression vector with integrin beta1 NPxY mutant (YYFF) tagged with mRuby2 |
| 215450 | pENTR2b-ITGB1(WT) | Gateway entry vector with integrin beta1 wild-type (WT) |
| 215451 | pENTR2b-ITGB1(YYFF) | Gateway entry vector with integrin beta1 NPxY mutant (YYFF) |
| 215452 | pDEST-Dok1-V2 | Expression vector with Dok1 tagged with the BiFC V2 fragment |
| 215455 | pENTR2b-Dok1-Clover | Gateway entry vector with Dok1 tagged with Clover |
| 215456 | pEF.DEST51-Dok1-Clover | Expression vector with Dok1 tagged with Clover |
| 215457 | pET28a-HaloTag-CNA35 | Encodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag. |
| 215458 | pEF.DEST51-Dok1 | Expression vector with Dok1 |
| 215459 | pENTR2b-Src(WT) | Gateway entry vector with Src wild-type (WT) |
| 215460 | pENTR2b-Src(Y527F) | Gateway entry vector with Src constitutively-active mutant (Y527F) |
| 215461 | pENTR2b-Src(K295R) | Gateway entry vector with Src kinase-dead mutant (K295R) |
| 215462 | pENTR2b-Src(E378G) | Gateway entry vector with Src constitutively-active mutant (E378G) |
| 215463 | pINDUCER20-Src(WT) | Dox-inducible lentiviral transfer vector with Src wild-type (WT) |
| 215464 | pINDUCER20-Src(Y527F) | Dox-inducible lentiviral transfer vector with Src constitutively-active mutant (Y527F) |
| 215465 | pINDUCER20-Src(K295R) | Dox-inducible lentiviral transfer vector with Src kinase-dead mutant (K295R) |
| 215466 | pINDUCER20-Src(E378G) | Dox-inducible lentiviral transfer vector with Src constitutively-active mutant (E378G) |
| 215472 | pENTR221-VPS35-mScarlet | Gateway entry vector with VPS35 tagged with mScarlet |
| 215473 | pENTR221-ANXA6-mScarlet | Gateway entry vector with Annexin A6 tagged with mScarlet |
| 215474 | pEF.DEST51-VPS35-mScarlet | Expression vector with VPS35 tagged with mScarlet |
| 215475 | pEF.DEST51-ANXA6-mScarlet | Expression vector with Annexin A6 tagged with mScarlet |
| 215476 | pENTR2b-PTPN11 | Gateway entry vector with Shp2 |
| 215477 | pENTR2b-PTPN12 | Gateway entry vector with PTP-PEST |
| 215515 | pENTR2b-PTPN11(D425A,C459S) | Gateway entry vector with a phosphatase-inactive Shp2 mutant (D425A,C459S) |
| 215516 | pENTR2b-PTPN12(D199A,C231S) | Gateway entry vector with a phosphatase-inactive PTP-PEST mutant (D199A,C231S) |
| 215517 | pINDUCER20-PTPN12 | Dox-inducible lentiviral transfer vector with PTP-PEST |
| 215518 | pINDUCER20-PTPN12(D199A,C231S) | Dox-inducible lentiviral transfer vector with a phosphatase-inactive PTP-PEST mutant (D199A,C231S) |
| 215519 | pENTR2b-PTPN11-Clover | Gateway entry vector with clover-tagged Shp2 |
| 215521 | pENTR2b-PTPN12-Clover | Gateway entry vector with clover-tagged PTP-PEST |
| 215522 | pENTR2b-PTPN12(D199A,C231S)-Clover | Gateway entry vector with a clover-tagged phosphatase-inactive PTP-PEST mutant (D199A,C231S) |
| 215523 | pEF.DEST51-PTPN11 | Expression vector with Shp2 |
| 215525 | pEF.DEST51-PTPN11-Clover | Expression vector with clover-tagged Shp2 |
| 215527 | pEF.DEST51-PTPN12-Clover | Expression vector with clover-tagged PTP-PEST |
| 215528 | pEF.DEST51-PTPN12(D199A,C231S)-Clover | Expression vector with a clover-tagged phosphatase-inactive PTP-PEST mutant (D199A,C231S) |
| 215529 | pENTR2b-Paxillin-EGFP | Gateway entry vector with EGFP-tagged paxillin |
| 215530 | pLenti6.3/TO/V5-DEST-Paxillin-EGFP | Lentiviral transfer vector with EGFP-tagged paxillin |
| 215534 | pDEST-mScarlet-SH3PXD2A | Expression vector with Tks5 tagged with N-terminal mScarlet |
| 215535 | pDEST-Dok1-mScarlet | Expression vector with Dok1 tagged with C-terminal mScarlet |
| 215536 | pLenti6.3/TO/V5-DEST-mScarlet | Lentiviral transfer vector to express the mScarlet fluorescent protein |
| 215537 | pENTR2b-EGFP | Gateway entry vector with the EGFP fluorescent protein |
| 215538 | pLenti6.3/TO/V5-DEST-EGFP | Lentiviral transfer vector to express the EGFP fluorescent protein |
| 215539 | pEF.DEST51-mTurquoise2 | Expression vector with the mTurquoise2 fluorescent protein |
| 215540 | pENTR2b-YPet | Gateway entry vector with the YPet fluorescent protein |
| 215541 | pEF.DEST51-YPet | Expression vector with the YPet fluorescent protein |
| 217453 | pDEST-ORF-mScarlet | Gateway destination vector with C-terminal mScarlet |
| 217454 | pDEST-mScarlet-ORF | Gateway destination vector with N-terminal mScarlet |
| 222591 | pENTR221-Illusia(YYEE) | Gateway entry vector with a phospho-mimetic mutant (YYEE) of the Illusia FRET reporter for integrin phosphorylation |
| 222594 | pPB.DEST-Illusia(YYEE) | Piggybac expression vector with a phospho-mimetic mutant (YYEE) of the Illusia FRET reporter for integrin phosphorylation |
| 222595 | pENTR221-Illusia(Y783F) | Gateway entry vector with a phospho-mutant (Y783F) variant of the Illusia FRET reporter for integrin phosphorylation |
| 222596 | pPB.DEST-Illusia(Y783F) | Piggybac expression vector with a phospho-mutant (Y783F) variant of the Illusia FRET reporter for integrin phosphorylation |
| 222597 | pENTR221-Illusia(Y795F) | Gateway entry vector with a phospho-mutant (Y795F) variant of the Illusia FRET reporter for integrin phosphorylation |
| 222598 | pPB.DEST-Illusia(Y795F) | Piggybac expression vector with a phospho-mutant (Y795F) variant of the Illusia FRET reporter for integrin phosphorylation |