Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks.
Frei MS, Sanchez SA, He X, Liu L, Schneider F, Wang Z, Hakozaki H, Li Y, Lyons AC, Rohm TV, Olefsky JM, Shi L, Schoneberg J, Fraser SE, Mehta S, Wang Y, Zhang J
Nat Biotechnol. 2025 Apr 21. doi: 10.1038/s41587-025-02642-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216488 | pcDNA3.1-HaloAKAR2.0-T2A-EGFP | Expression of chemigenetic PKA biosensor (high basal brightness, medium dynamic range) and EGFP transfection marker in mammalian cells |
| 216489 | pcDNA3.1-HaloAKAR2.1-T2A-EGFP | Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells |
| 216490 | pcDNA3.1-HaloAKAR2.2-T2A-EGFP | Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells |
| 216491 | pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX | Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and PM targeted EGFP in mammalian cells |
| 216492 | pcDNA3.1-HaloAKAR2.1-CLC | Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) targeted to clathrin coated pits |
| 216493 | pcDNA3.1-HaloCKAR2.2-T2A-EGFP | Expression of chemigenetic PKC biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells |
| 216494 | pcDNA3.1-HaloAktKAR2.2-T2A-EGFP | Expression of chemigenetic Akt biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells |
| 216495 | pcDNA3.1-HaloEKAR2.2-T2A-EGFP | Expression of chemigenetic ERK biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells |