Efficient gene activation in plants by the MoonTag programmable transcriptional activator.
Casas-Mollano JA, Zinselmeier MH, Sychla A, Smanski MJ
Nucleic Acids Res. 2023 Jul 21;51(13):7083-7093. doi: 10.1093/nar/gkad458.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202005 | pMC-J6-dCas9-10XGP41-J9 | MoClo level 0 dCas9-10XGP41 coding sequence module |
| 202006 | pMC-J6-dCas9-24XGP41-J9 | MoClo level 0 dCas9-24XGP41 coding sequence module |
| 202007 | pMC-J6-NbGP41-sfGFP-VP64-GB1-J9 | MoClo level 0 NbGP41-sfGFP-VP64-GB1 coding sequence module |
| 202009 | pMOD_A-CmYLCV-dCas9-24XGP41 | Moclo level 1 - Module A, Promoter: CmYLCV, Gene: dCas9-24XGP41, Terminator: HSP |
| 202010 | pMOD_D-CmYLCV-NbGP41P-sfGFP-VP64-GB1 | Moclo level 1 - Module D, Promoter: CmYLCV, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: ZmUBI |
| 202011 | pMOD_A-AtUBI10-dCas9-24XGP41 | Moclo level 1 - Module A, Promoter: AtUBI10, Gene: dCas9-24XGP41, Terminator: HSP |
| 202012 | pMOD_D-AtUBI10-NbGP41-sfGFP-VP64-GB1 | Moclo level 1 - Module D, Promoter: AtUBI10, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: pea rbcsE9 |
| 202014 | pMT1-24X-NOG(CmYLCV) | T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter. |
| 202016 | pMT1-24X-NOG(AtUBI10) | T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter. |
| 234370 | pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC | T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs |
| 234371 | pMT1-13X-NOG(AtUBI10)-MTAP-LUC | T-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG) |
| 234372 | pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC | T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs |