Development of multiplexed orthogonal base editor (MOBE) systems.
Cowan QT, Gu S, Gu W, Ranzau BL, Simonson TS, Komor AC
Nat Biotechnol. 2024 May 21. doi: 10.1038/s41587-024-02240-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 219934 | nCas9-NG_P2A-mCherry | Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems |
| 219935 | nCas9-SpRY_P2A-mCherry | Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems |
| 219936 | HiFi-nCas9-NG_P2A-mCherry | Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems |
| 219937 | apt-ABE8e | Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker |
| 219938 | apt-ABE8.20 | Mammalian expression of apt-ABE8.20 protein (TadA8.20_32aa-linker_Com) |
| 219939 | apt-CBE-3'end | Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker |
| 219940 | apt-CBE-SL3 | Mammalian expression of apt-CBE-SL3 protein (MCP_16aa-linker_evoAPOBEC1) with P2A-EGFP marker |
| 219942 | MOBE2 | Mammalian expression of MOBE2 protein (apt-CBE-3'end_PT2A_apt-ABE8.20) |
| 219943 | MOBE3 | Mammalian expression of MOBE3 protein (apt-CBE-SL3_PT2A_apt-ABE8e) |
| 219944 | MOBE4 | Mammalian expression of MOBE4 protein (apt-CBE-SL3_PT2A_apt-ABE8.20) |
| 219945 | Fluorescent reporter for MOBE1-2 | Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2 |
| 219946 | Fluorescent reporter for MOBE3-4 | Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4 |
| 219947 | 3'-com-gRNA GG backbone (MOBE1-4) | Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com |
| 219948 | 3'MS2-gRNA GG backbone (MOBE1-2) | Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2 |
| 219949 | SL3-MS2-gRNA GG backbone (MOBE3-4) | Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2 |