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Design of highly functional genome editors by modelling CRISPR-Cas sequences.

Ruffolo JA, Nayfach S, Gallagher J, Bhatnagar A, Beazer J, Hussain R, Russ J, Yip J, Hill E, Pacesa M, Meeske AJ, Cameron P, Madani A
Nature. 2025 Jul 30. doi: 10.1038/s41586-025-09298-z. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
221565OpenCRISPR-1Express OpenCRISPR-1 nuclease in human cells using a CMV promoter
221566OpenCRISPR-1 sgRNA-16nt stemExpress OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.
221567OpenCRISPR-1 sgRNA-12nt stemExpress OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

Antibodies from Article