Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy.
Basak B, Holzbaur ELF
Nat Commun. 2025 Aug 9;16(1):7367. doi: 10.1038/s41467-025-62379-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221655 | LAMP1-Halo | Lysosomal marker LAMP1 tagged C-terminally with Halo tag |
| 221656 | mCherry-LC3 | Autophagosome marker LC3 tagged N-terminally with mCherry |
| 221657 | mCherry-Rubicon | Rubicon tagged N-terminally with mCherry |
| 221658 | mCherry-Rubicon-CGHL | Rubicon mutated at four residues in the RH domain to disrupt Rab7 binding, tagged at the N-terminus with mCherry |
| 221659 | EGFP-Rubicon | Rubicon tagged N-terminally with EGFP; clone a gift from the Hurley Lab at UC Berkeley |