Human DNA polymerase eta is regulated by mutually exclusive mono-ubiquitination and mono-NEDDylation.
Moreno NC, Korchak EJ, Latancia MT, D'Orlando DA, Adegbenro T, Barnes RP, Bezsonova I, Woodgate R, Ashton NW
Nucleic Acids Res. 2026 Feb 5;54(4):gkag098. doi: 10.1093/nar/gkag098.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221858 | pcDNA3.1(+)-N-DYK_WT PCNA | Expresses WT PCNA with a FLAG tag in mammalian cells |
| 221859 | pcDNA3.1(+)-N-HA_NEDD8 | Expresses WT unprocessed NEDD8 with a HA tag in mammalian cells |
| 221860 | pCMV6-AN-DDK_WT POLH_ΔGG NEDD8 | Expresses FLAG-tagged WT POLH fused to ΔGG NEDD8 in mammalian cells |
| 221861 | pCMV6-AN-DDK_WT POLH_ΔGG Ubiquitin | Expresses FLAG-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells |
| 221862 | pCMV6-AN-DDK_K682A POLH | Expresses POLH mutated at K682A with a FLAG tag in mammalian cells |
| 221863 | pCMV6-AN-DDK_K709A POLH | Expresses POLH mutated at K709A with a FLAG tag in mammalian cells |
| 221864 | pCMV6-AN-DDK_K682A_K709A POLH | Expresses POLH mutated at K682A and K709A with a FLAG tag in mammalian cells |
| 221865 | pCMV6-AN-DDK_K682A_K686A_K694A_K709A POLH | Expresses POLH mutated at K682A, K686A, K694A and K709A with a FLAG tag in mammalian cells |
| 221866 | pET-28b(+)_N-His_NEDD8 | Expresses mature WT human NEDD8 with a HIS tag in E. coli |
| 221867 | pEGFP-C1_N-NLS | Empty backbone for expressing GFP-fusion proteins in mammalian cells with a nuclear localization signal (NLS). |
| 221868 | pEGFP-C1_N-NLS_WT POLH | Expresses WT POLH with an N-terminal NLS-GFP tag in mammalian cells |
| 221869 | pEGFP-C1_N-NLS_POLH_ΔGG ubiquitin | Expresses GFP-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells |
| 221870 | pEGFP-C1_N-NLS_POLH_ΔGG NEDD8 | Expresses GFP-tagged WT POLH fused to ΔGG NEDD8 in mammalian cells |
| 221871 | pEGFP-C1_N-NLS_D652A POLH | Expresses D652A POLH with an N-terminal NLS-GFP tag in mammalian cells |
| 221872 | pEGFP-C1_N-NLS_K682A_K686A_K694A_K709A POLH | Expresses K682A_K686A_K694A_K709A POLH with an N-terminal NLS-GFP tag in mammalian cells |
| 221873 | pEGFP-C1_N-NLS_L704A_F707A_F708A POLH | Expresses PIP box-mutated POLH with an N-terminal NLS-GFP tag in mammalian cells |
| 221874 | pcDNA3.1(+)-N-DYK_K164R PCNA | Expresses K164R PCNA with a FLAG tag in mammalian cells |
| 221875 | pcDNA3.1(+)-N-DYK_K164R PCNA_ΔGG ubiquitin | Expresses FLAG-tagged K164R PCNA fused with ΔGG Ubiquitin in mammalian cells |
| 221897 | pCMV6-AN-DDK_WT POLH | Expresses WT POLH with a FLAG tag in mammalian cells |
| 222006 | pCMV6-AN-DDK_D652A POLH | Expresses D652A POLH with a FLAG tag in mammalian cells |
| 246283 | pCMV6-AN-DDK_L704A_F707A_F708A (PIP2*) POLH | Expresses L704A_F707A_F708A POLH with a FLAG tag in mammalian cells |
| 246405 | pCMV6-AN-DDK_NLS~WT POLH | Expresses WT POLH with an N-terminal NLS~FLAG tag in mammalian cells |
| 246406 | pCMV6-AN-DDK_ NLS~K682A_K686A_K694_K709A (4KA) POLH | Expresses K682A_K686A_K694_K709A POLH with an N-terminal NLS~FLAG tag in mammalian cells |
| 246407 | pCMV6-AN-DDK_ NLS~K682R_K686R_K69R_K709R (4KR) POLH | Expresses K682R_K686R_K69R_K709R POLH with an N-terminal NLS~FLAG tag in mammalian cells |
| 246408 | pEGFP-C1_NLS_ K682R_K686R_K69R_K709R POLH (4KR) | Expresses K682R_K686R_K694R_K709R POLH with an N-terminal NLS-GFP tag in mammalian cells |
| 246409 | pEGFP-C1_NLS_ K682A_K686A_K694A_K709A (4KA) POLH_deltaGG Ubiquitin | Expresses GFP-tagged K682A_K686A_K694A_K709A POLH fused to ΔGG ubiquitin in mammalian cells |
| 246410 | pEGFP-C1_NLS_ K682R_K686R_K694R_K709R (4KR) POLH_deltaGG Ubiquitin | Expresses GFP-tagged K682R_K686R_K694R_K709R POLH fused to ΔGG ubiquitin in mammalian cells |
| 246411 | pCMV6-AN-HA_ WT POLH_deltaGG Ubiquitin | Expresses HA-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells |
| 246412 | pCMV6-AN-HA_WT POLH_deltaGG NEDD8 | Expresses HA-tagged WT POLH fused to ΔGG NEDD8 in mammalian cells |
| 250151 | pEGFP-C1_NLS_ K682R_K686R_K694R_K709R (4KR) POLH_deltaGG NEDD8 | Expresses GFP-tagged K682R_K686R_K694R_K709R POLH fused to ΔGG NEDD8 in mammalian cells |