Building Synthetic Biosensors Using Red Blood Cell Proteins.
Dolberg TB, Gunnels TF, Ling T, Sarnese KA, Crispino JD, Leonard JN
ACS Synth Biol. 2024 Apr 19;13(4):1273-1289. doi: 10.1021/acssynbio.3c00754. Epub 2024 Mar 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 222875 | L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135 | Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold. |
| 222876 | L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138 | Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold. |
| 222877 | L2092-GPA-FullBRET-Retrovirus-TD142 | Retroviral vector encoding positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, RH linker, CyOFP1. |
| 222878 | L2096-GPA-BRET-Biosensor-Retrovirus-TD146 | Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold. |
| 222879 | L2108-FRB-GPA-20-114 | Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114. |
| 222880 | L2109-FRB-GPA-20-11S | Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S. |
| 222881 | L2110-FKBP-GPA-20-114 | Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114. |
| 222882 | L2111-FKBP-GPA-20-11S | Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S. |
| 222883 | L2116-FRB-GPA-114-RH-CyOFP1 | Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein. |
| 222884 | L2117-FKBP-GPA-114-RH-CyOFP1 | Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein. |
| 222885 | L2125-FRB-GPA-NLuc-CyOFP1 | Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1. |
| 222886 | L2127-FRB-GPA-114-20-CyOFP1 | Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein. |
| 222887 | L2128-FRB-GPA-114-10-CyOFP1 | Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein. |
| 222888 | L2129-FRB-GPA-114-5-CyOFP1 | Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein. |
| 222889 | L2130-FKBP-GPA-114-20-CyOFP1 | Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein. |
| 222890 | L2131-FKBP-GPA-114-10-CyOFP1 | Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein. |
| 222891 | L2132-FKBP-GPA-114-5-CyOFP1 | Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein. |
| 222893 | L2179-FRB-GPA-6-GFPS | Biosensor chain detecting rapamycin and responding with split GFP reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 6 GS linker, split GFP fragment small. |
| 222894 | L2373-FRB-GPA-20-114 | Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114. |
| 222895 | L2374-FRB-GPA-20-11S | Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S. |
| 222896 | L2376-FKBP-GPA-20-114 | Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114. |
| 222897 | L2375-FKBP-GPA-20-11S | Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S. |
| 222898 | L2377-FRB-GPA(V84R)-20-114 | Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114. |
| 222899 | L2378-FRB-GPA(V84R)-20-11S | Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S. |
| 222900 | L2380-FKBP-GPA(V84R)-20-114 | Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114 |
| 222901 | L2379-FKBP-GPA(V84R)-20-11S | Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S |
| 222902 | L2383-FRB-GPA-NLuc | Positive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase. |