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A sensitive orange fluorescent calcium ion indicator for imaging neural activity

Aggarwal A, Baker HA, Durst CD, Chen I-W, de Chambrier P, Gonzales JM, Marvin JS, Vandal M, Lundberg T, Sakoi K, Patel RH, Wang C-Y, Visser F, Fouad Y, Sunil S, Wiens M, Terai T, Takahashi-Yamashiro K, Thompson RJ, Brown TA, Nasu Y, Nguyen MD, Gordon GRJ, McFarlane S, Podgorski K, Holtmaat A, Campbell RE, Lohman AW
bioRxiv 2025.07.28.667269; doi: https://doi.org/10.1101/2025.07.28.667269 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
224932pRSET OCaMPExpresses OCaMP, an orange calcium indicator, in bacteria. Contains T7 promoter and RSET leader sequence peptide (His tag, T7 leader, Xpress epitope)
224934pAAV-hSYN1-NES-OCaMP-WPREpAAV (non-flex) for expressing OCaMP, an orange calcium indicator, in mammalian cells and in vivo. Contains hSyn promoter and RSET leader sequence peptide
224936pAAV-EF1a-FLEX(FLP)-OCaMP-WPREpAAV vector for flippase(FLP)-dependent OCaMP (orange calcium indicator) expression under the control of EF1a promoter

Antibodies from Article