A sensitive orange fluorescent calcium ion indicator for imaging neural activity
Aggarwal A, Baker HA, Durst CD, Chen I-W, de Chambrier P, Gonzales JM, Marvin JS, Vandal M, Lundberg T, Sakoi K, Patel RH, Wang C-Y, Visser F, Fouad Y, Sunil S, Wiens M, Terai T, Takahashi-Yamashiro K, Thompson RJ, Brown TA, Nasu Y, Nguyen MD, Gordon GRJ, McFarlane S, Podgorski K, Holtmaat A, Campbell RE, Lohman AW
bioRxiv 2025.07.28.667269; doi: https://doi.org/10.1101/2025.07.28.667269
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224932 | pRSET OCaMP | Expresses OCaMP, an orange calcium indicator, in bacteria. Contains T7 promoter and RSET leader sequence peptide (His tag, T7 leader, Xpress epitope) |
| 224934 | pAAV-hSYN1-NES-OCaMP-WPRE | pAAV (non-flex) for expressing OCaMP, an orange calcium indicator, in mammalian cells and in vivo. Contains hSyn promoter and RSET leader sequence peptide |
| 224936 | pAAV-EF1a-FLEX(FLP)-OCaMP-WPRE | pAAV vector for flippase(FLP)-dependent OCaMP (orange calcium indicator) expression under the control of EF1a promoter |
| 229853 | pAAV-hSyn-FLEX(cre)-OCaMP-WPRE | pAAV vector for Cre-dependent OCaMP (orange calcium indicator) expression under the control of hSyn promoter |