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A Cas12a Toolbox for Rapid and Flexible Group B Streptococcus Genomic Editing and CRISPRi.

Hillebrand GH, Carlin SC, Giacobe EJ, Stephenson HA, Collins J, Hooven TA
Mol Microbiol. 2025 Nov;124(5):449-461. doi: 10.1111/mmi.70022. Epub 2025 Sep 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
223200pGBSedit ShamGroup B Streptococcus Efficient Genome Editing with Modular crRNA
223201pGBScrispri ShamGroup B Streptococcus inducible dCas12 CRISPRi with Modular crRNA
223202pGBScomp HighGroup B Streptococcus High Expression Modular Complementation Vector
223203pGBScomp LowGroup B Streptococcus Low Expression Modular Complementation Vector

Antibodies from Article