Rapid discovery of monoclonal antibodies by microfluidics-enabled FACS of single pathogen-specific antibody-secreting cells.
Fischer K, Lulla A, So TY, Pereyra-Gerber P, Raybould MIJ, Kohler TN, Yam-Puc JC, Kaminski TS, Hughes R, Pyeatt GL, Leiss-Maier F, Brear P, Matheson NJ, Deane CM, Hyvonen M, Thaventhiran JED, Hollfelder F
Nat Biotechnol. 2025 Jun;43(6):960-970. doi: 10.1038/s41587-024-02346-5. Epub 2024 Aug 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195000 | pExp-His-zBasic-RBD-Avi | Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag |
| 195002 | pExp-RBD-CHis | Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag |