PUFFFIN: an ultra-bright, customisable, single-plasmid system for labelling cell neighbourhoods.
Lebek T, Malaguti M, Boezio GL, Zoupi L, Briscoe J, Elfick A, Lowell S
EMBO J. 2024 Jul 12. doi: 10.1038/s44318-024-00154-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 223540 | pTL5_cHS4-CAG-s36GFPmNG-IRES-PuroR-p2AmCherry3NLS-polyA-cHS4 | PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, strong constitutive promoter CAG, insulated by cHS4 for random integration, puromycin resistance for selection in mammalian cells |
| 223541 | pTL50_cHS4-CAG-s36GFPmNG-t2A-PuroR-p2A-BFP-polyAcHS4 | PUFFFIN cassette with s36GFP-mNeonGreen and tagBFP, strong constitutive promoter CAG, insulated by cHS4 for random integration, puromycin resistance for selection in mammalian cells |
| 223542 | pTL51_cHS4-CAG-s36GFPHaloTag-IRES-PuroR-p2AmCherry3NLS-polyA-cHS4 | PUFFHalo cassette with s36GFP-HaloTag and mCherry-3NLS, strong constitutive promoter CAG, insulated by cHS4 for random integration, puromycin resistance for selection in mammalian cells |
| 223544 | pTL52_pyCAG-s36GFPHaloTag-IRES-mCherry3NLS-polyA | Minimal PUFFHalo cassette with s36GFP-HaloTag and mCherry-3NLS, strong constitutive promoter CAG, no insulation or mammalian selection gene |
| 223545 | pTL53_pyCAG-s36GFPmNG-IRES-mCherry3NLS-polyA | Minimal PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, separated by IRES, strong constitutive promoter CAG, no insulation or mammalian selection gene |
| 223546 | pTL54_pyCAG-s36GFPmNG-t2A-mCherry3NLS-polyA | Minimal PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, separated by t2A self-cleaving peptide, strong constitutive promoter CAG, no insulation or mammalian selection gene |
| 223547 | pLenti6_cppt-CMV-Puffling-opre | Minimal PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, separated by t2A self-cleaving peptide, strong constitutive promoter CMV, for lentivirus |