Synergy between regulatory elements can render cohesin dispensable for distal enhancer function.
Hansen KL, Adachi AS, Braccioli L, Kadvani S, Boileau RM, Martinovic M, Pokorny B, Shah R, Anderson EC, Zhang K, Carel I, Bonitto K, Blelloch R, Fudenberg G, de Wit E, Nora EP
Science. 2025 Nov 27:eadt4221. doi: 10.1126/science.adt4221.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 224546 | pEN243-EN1953 | Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl |
| 224547 | pEA038 | Blast-T2A-2xHA-FKPB12(dTagDegron) mouse Nipbl N-term targeting vector |
| 224548 | pKH032 | 2A-3xNLS-2xClover mouse Car2 C-term targeting vector |
| 224549 | pKH040 | 2A-3xNLS-tdTomato mouse Car2 C-term targeting vector |
| 224550 | pEN243-KH217 | Cas9-2A-puro and sgRNA targeting the STOP codon mouse Car2 |