Genetically Encoded Fluorescence Barcodes Allow for Single-Cell Analysis via Spectral Flow Cytometry.
Lu X, Pritko DJ, Abravanel ME, Huggins JR, Ogunleye O, Biswas T, Ashy KC, Woods SK, Livingston MWT, Blenner MA, Birtwistle MR
ACS Synth Biol. 2025 May 16;14(5):1533-1548. doi: 10.1021/acssynbio.4c00807. Epub 2025 May 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227193 | pMuSIC pool v1.0 | Two-way barcodes from 18 fluorescent proteins for mammalian expression. Can be used for clone tracing, or for pairing with perturbations for single cell resolution in spectral detection measurements. |
| 228594 | pReceiver | Mammalian expression of a single fluorescent protein, for MuSIC barcode construction |
| 228595 | pR-EBFP2 | Mammalian expression of EBFP2, for MuSIC barcode construction |
| 228596 | pR-mTagBFP2 | Mammalian expression of mTagBFP2, for MuSIC barcode construction |
| 228597 | pR-mT-Sapphire | Mammalian expression of mT-Sapphire, for MuSIC barcode construction |
| 228598 | pR-mAmetrine | Mammalian expression of mAmetrine, for MuSIC barcode construction |
| 228599 | pR-mCerulean3 | Mammalian expression of mCerulean3, for MuSIC barcode construction |
| 228600 | pR-LSSmOrange | Mammalian expression of LSSmOrange, for MuSIC barcode construction |
| 228601 | pR-mBeRFP | Mammalian expression of mBeRFP, for MuSIC barcode construction |
| 228602 | pR-mTFP1 | Mammalian expression of mTFP1, for MuSIC barcode construction |
| 228603 | pR-EGFP | Mammalian expression of EGFP, for MuSIC barcode construction |
| 228604 | pR-CyOFP1 | Mammalian expression of CyOFP1, for MuSIC barcode construction |
| 228605 | pR-mClover3 | Mammalian expression of mClover3, for MuSIC barcode construction |
| 228606 | pR-mVenus | Mammalian expression of mVenus, for MuSIC barcode construction |
| 228607 | pR-mPapaya | Mammalian expression of mPapaya, for MuSIC barcode construction |
| 228608 | pR-mOrange2 | Mammalian expression of mOrange2, for MuSIC barcode construction |
| 228609 | pR-mRuby3 | Mammalian expression of mRuby3, for MuSIC barcode construction |
| 228610 | pR-mKate2 | Mammalian expression of mKate2, for MuSIC barcode construction |
| 228611 | pR-mCardinal | Mammalian expression of mCardinal, for MuSIC barcode construction |
| 228612 | pR-miRFP670 | Mammalian expression of miRFP670, for MuSIC barcode construction |
| 228613 | pMulti | Mammalian expression of a single fluorescent protein and extra insert, for MuSIC barcode construction |
| 228614 | pM-EBFP2 | Mammalian expression of EBFP2 and extra insert, for MuSIC barcode construction |
| 228615 | pM-mTagBFP2 | Mammalian expression of mTagBFP2 and extra insert, for MuSIC barcode construction |
| 228616 | pM-mT-Sapphire | Mammalian expression of mT-Sapphire and extra insert, for MuSIC barcode construction |
| 228617 | pM-mAmetrine | Mammalian expression of mAmetrine and extra insert, for MuSIC barcode construction |
| 228618 | pM-mCerulean3 | Mammalian expression of mCerulean3 and extra insert, for MuSIC barcode construction |
| 228619 | pM-LSSmOrange | Mammalian expression of LSSmOrange and extra insert, for MuSIC barcode construction |
| 228620 | pM-mBeRFP | Mammalian expression of mBeRFP and extra insert, for MuSIC barcode construction |
| 228621 | pM-mTFP1 | Mammalian expression of mTFP1 and extra insert, for MuSIC barcode construction |
| 228622 | pM-EGFP | Mammalian expression of EGFP and extra insert, for MuSIC barcode construction |
| 228623 | pM-CyOFP1 | Mammalian expression of CyOFP1 and extra insert, for MuSIC barcode construction |
| 228624 | pM-mClover3 | Mammalian expression of mClover3 and extra insert, for MuSIC barcode construction |
| 228625 | pM-mVenus | Mammalian expression of mVenus and extra insert, for MuSIC barcode construction |
| 228626 | pM-mPapaya | Mammalian expression of mPapaya and extra insert, for MuSIC barcode construction |
| 228627 | pM-mOrange2 | Mammalian expression of mOrange2 and extra insert, for MuSIC barcode construction |
| 228628 | pM-mRuby3 | Mammalian expression of mRuby3 and extra insert, for MuSIC barcode construction |
| 228629 | pM-mKate2 | Mammalian expression of mKate2 and extra insert, for MuSIC barcode construction |
| 228630 | pM-mCardinal | Mammalian expression of mCardinal and extra insert, for MuSIC barcode construction |
| 228631 | pM-miRFP670 | Mammalian expression of miRFP670 and extra insert, for MuSIC barcode construction |