Genome organization around nuclear speckles drives mRNA splicing efficiency.
Bhat P, Chow A, Emert B, Ettlin O, Quinodoz SA, Strehle M, Takei Y, Burr A, Goronzy IN, Chen AW, Huang W, Ferrer JLM, Soehalim E, Goh ST, Chari T, Sullivan DK, Blanco MR, Guttman M
Nature. 2024 May;629(8014):1165-1173. doi: 10.1038/s41586-024-07429-6. Epub 2024 May 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235086 | BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron | GFP splicing reporter with CORO1B intron |
| 235087 | BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron | GFP splicing reporter with FRG1 intron |
| 235088 | pCAG-MCP-SRSF1-V5-mCherry | SRSF1 mCherry fusion protein with MCP domain |
| 235089 | pCAG-SpyTag-Flag-SRSF1-V5-mCherry | SRSF1 mCherry fusion protein without MCP domain |
| 235090 | pCAG-MCP-SRSF3-V5-mCherry | SRSF3 mCherry fusion protein with MCP domain |
| 235091 | pCAG-SpyTag-Flag-SRSF3-V5-mCherry | SRSF3 mCherry fusion protein without MCP domain |
| 235093 | pCAG-MCP-Lbr-V5-mCherry | Lbr mCherry fusion protein with MCP domain |
| 235094 | pCAG-SpyTag-Flag-Lbr-V5-mCherry | Lbr mCherry fusion protein without MCP domain |
| 235095 | pCAG-MCP-dNS-SRRM1-V5-mCherry | SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein with MCP domain |
| 235096 | pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry | SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein without MCP domain |