AMPK targets PDZD8 to trigger carbon source shift from glucose to glutamine.
Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 227821 | pBOBI C-MYC KGA F7 | To amplify the virus that expresses C-MYC KGA F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8 |
| 227822 | pGEX KGA F7 | To express in E. coli KGA F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8 |
| 227823 | pBOBI C-GFP PDZD8 | To amplify the virus that expresses C-GFP PDZD8 |
| 227824 | pBOBI NT-RFP-PDZD8-CT-GFP | To amplify the virus that expresses NT-RFP-PDZD8-CT-GFP |
| 227825 | pBOBI C-GFP PDZD8 T527A | To amplify the virus that expresses C-GFP PDZD8 T527A |
| 227826 | pBOBI nt-rfp-PDZD8 T527A ct-gfp | To amplify the virus that expresses nt-rfp-PDZD8 T527A ct-gfp |
| 227827 | pBOBI C-APEX PDZD8 | To amplify the virus that expresses C-APEX PDZD8 |
| 227839 | pcDNA 3.3 C-FLAG PDZD8-T527A | Used for transfection of cells for expression of C-FLAG PDZD8-T527A |
| 227841 | pcDNA 3.3 C-MYC GAC-WT | Used for transfection of cells for expression of N-MYC GAC-WT |
| 227843 | pcDNA 3.3 C-MYC KGA-WT | Used for transfection of cells for expression of N-MYC KGA-WT |
| 227844 | pcDNA 3.3 N-MYC pdzd-8 | Used for transfection of cells for expression of C-MYC pdzd-8 |
| 227845 | pET-28a GAC-WT | To express in E. coli GAC-WT |
| 227846 | pET-28a KGA-WT | To express in E. coli KGA-WT |
| 227849 | pGEX4T-1 PDZD8-WT | To express in E. coli PDZD8-WT |
| 227850 | pGEX4T-1 PDZD8-T527A | To express in E. coli PDZD8-T527A |
| 227851 | pET-28a PDZD8-T527D | To express in E. coli PDZD8-T527D |
| 227852 | pET-28a PDZD8-T527E | To express in E. coli PDZD8-T527E |
| 227853 | pGEX4T-1 AMPK αKD(27-290aa) | To express in E. coli AMPK αKD(27-290aa) |
| 227854 | pGEX4T-1 PDZD8-CT | To express in E. coli PDZD8-CT |
| 227856 | pcDNA 3.3 C-FLAG PDZD8(367-1154aa) | Used for transfection of cells for expression of C-FLAG PDZD8 (367-1154aa) |
| 227857 | pcDNA 3.3 C-FLAG PDZD8(295-1154aa) | Used for transfection of cells for expression of C-FLAG PDZD8 (295-1154aa) |
| 227858 | pcDNA 3.3 C-FLAG PDZD8(91-1154aa) | Used for transfection of cells for expression of C-FLAG PDZD8 (91-1154aa) |
| 227859 | pcDNA 3.3 C-FLAG PDZD8(66-1154aa) | Used for transfection of cells for expression of C-FLAG PDZD8 (66-1154aa) |
| 227860 | pcDNA 3.3 C-MYC PDZD8(1-839aa) | Used for transfection of cells for expression of C-MYC PDZD8 (1-839aa) |
| 227861 | pcDNA 3.3 C-MYC PDZD8(1-449aa) | Used for transfection of cells for expression of C-MYC PDZD8 (1-839aa) |
| 227862 | pcDNA 3.3 C-MYC PDZD8(1-294aa) | Used for transfection of cells for expression of C-MYC PDZD8 (1-294aa) |
| 227863 | pcDNA 3.3 C-MYC PDZD8(1-90aa) | Used for transfection of cells for expression of C-MYC PDZD8 (1-90aa) |
| 227864 | pcDNA 3.3 C-MYC PDZD8(450-839aa) | Used for transfection of cells for expression of C-MYC PDZD8 (450-839aa) |
| 227865 | pcDNA 3.3 C-MYC PDZD8(367-449aa) | Used for transfection of cells for expression of C-MYC PDZD8 (367-449aa) |
| 227866 | pcDNA 3.3 C-MYC PDZD8(91-294aa) | Used for transfection of cells for expression of C-MYC PDZD8 (91-294aa) |
| 227867 | pcDNA 3.3 C-MYC PDZD8(367-839aa) | Used for transfection of cells for expression of C-MYC PDZD8 (367-839aa) |
| 227870 | pcDNA 3.3 C-MYC PDZD8(ΔSMP 91-294aa) | Used for transfection of cells for expression of C-MYC PDZD8 (with deletion of SMP aa 91-294) |
| 227871 | pcDNA 3.3 C-MYC PDZD8(ΔPDZ 367-449aa) | Used for transfection of cells for expression of C-MYC PDZD8 (with deletion of PDZ aa 367-449) |
| 227872 | pcDNA 3.3 C-MYC PDZD8(Δ451-839aa) | Used for transfection of cells for expression of C-MYC PDZD8 (with deletion of aa 451-839) |
| 227874 | pcDNA 3.3 N-MYC CYTSB | Used for transfection of cells for expression of C-MYC CYTSB |
| 227875 | pcDNA 3.3 N-MYC LIMA1 | Used for transfection of cells for expression of C-MYC LIMA1 |
| 227876 | pcDNA 3.3 N-MYC FLNA | Used for transfection of cells for expression of C-MYC FLNA |
| 227877 | pcDNA 3.3 N-MYC PDHA1 | Used for transfection of cells for expression of C-MYC PDHA1 |
| 227878 | pcDNA 3.3 N-MYC RMDN3 | Used for transfection of cells for expression of C-MYC RMDN3 |
| 227879 | pcDNA 3.3 N-MYC EPHA2 | Used for transfection of cells for expression of C-MYC EPHA2 |
| 227880 | pcDNA 3.3 N-MYC SYEM | Used for transfection of cells for expression of C-MYC SYEM |
| 227881 | pcDNA 3.3 N-MYC ES8L3 | Used for transfection of cells for expression of C-MYC ES8L3 |
| 227882 | pcDNA 3.3 N-MYC TB182 | Used for transfection of cells for expression of C-MYC TB182 |
| 227885 | pcDNA 3.3 C-MYC pdzd-8(536A) | Used for transfection of cells for expression of C-MYC pdzd-8(536A) |