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AMPK targets PDZD8 to trigger carbon source shift from glucose to glutamine.

Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
227821pBOBI C-MYC KGA F7To amplify the virus that expresses C-MYC KGA F7 with 33 residues mutated to alanine at the interface for interacting with PDZD8
227823pBOBI C-GFP PDZD8To amplify the virus that expresses C-GFP PDZD8
227824pBOBI NT-RFP-PDZD8-CT-GFPTo amplify the virus that expresses NT-RFP-PDZD8-CT-GFP
227825pBOBI C-GFP PDZD8 T527ATo amplify the virus that expresses C-GFP PDZD8 T527A
227827pBOBI C-APEX PDZD8To amplify the virus that expresses C-APEX PDZD8
227844pcDNA 3.3 N-MYC pdzd-8Used for transfection of cells for expression of C-MYC pdzd-8
227845pET-28a GAC-WTTo express in E. coli GAC-WT
227846pET-28a KGA-WTTo express in E. coli KGA-WT
227851pET-28a PDZD8-T527DTo express in E. coli PDZD8-T527D
227852pET-28a PDZD8-T527ETo express in E. coli PDZD8-T527E
227866pcDNA 3.3 C-MYC PDZD8(91-294aa)Used for transfection of cells for expression of C-MYC PDZD8 (91-294aa)
227875pcDNA 3.3 N-MYC LIMA1Used for transfection of cells for expression of C-MYC LIMA1
227876pcDNA 3.3 N-MYC FLNAUsed for transfection of cells for expression of C-MYC FLNA
227881pcDNA 3.3 N-MYC ES8L3Used for transfection of cells for expression of C-MYC ES8L3

Antibodies from Article