STRAIGHT-IN Dual: a platform for dual, single-copy integrations of DNA payloads and gene circuits into human induced pluripotent stem cells.
Blanch-Asensio A, Ploessl DS, Wang NB, Mummery CL, Galloway KE, Davis RP
bioRxiv [Preprint]. 2024 Oct 17:2024.10.17.616637. doi: 10.1101/2024.10.17.616637.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 229776 | CLYBL_Bxb1-GA_LP_Dual_TC | CLYBL targeting construct with the Bxb1-GA landing pad cassette. |
| 229777 | Bxb1-GT_CAG_3xTurboGFP-NLS | Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies) |
| 229778 | Bxb1-GT_CAG-ITPKA-mScarlet-I | Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter |
| 229779 | Bxb1-GA Donor_hEF1a | Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker |
| 229780 | Bxb1-GA Donor_CAG | Bxb1-GA donor plasmid for the cloning of payloads <20 kb with CAG promoter for expressing landing pad enrichment marker |
| 229781 | Bxb1-GA_CAG-TurboGFP | Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP |
| 229782 | Bxb1-GA_CAG-ITPKA-3x-mScarlet-I | Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter (3 copies) |
| 229783 | Bxb1-GT_AIO-TetOn_Down-Tandem Donor | Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty) |
| 229784 | Bxb1-GT_AIO-TetOn_Divergent Donor | Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty) |
| 229785 | Bxb1-GA_AIO-TetOn_Down-Tandem Donor | Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty) |
| 229786 | Bxb1-GA_AIO-TetOn_Divergent Donor | Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty) |
| 229787 | Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor | Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty) |
| 229788 | Bxb1-GA_AIO-GZV-SynZiFTR_mGL donor | Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible mGreenLantern expression |
| 229789 | Bxb1-GA_AIO-GZV-SynZiFTR_mNgn2 donor | Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of mouse neurogenin 2 |
| 229790 | Bxb1-GA_AIO-TetOn_mScarlet_Convergent | Bxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression |
| 229791 | Bxb1-GA_AIO-TetOn_mScarlet_Divergent | Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression |
| 229792 | Bxb1-GA_AIO-TetOn_mScarlet_Down-Tandem | Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression |
| 229793 | Bxb1-GA_AIO-TetOn_mScarlet_Up-Tandem | Bxb1-GA donor plasmid with upstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression |
| 229794 | Bxb1-GT_AIO-TetOn_mScarlet_Down-Tandem | Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression |
| 229795 | Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem | Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2 |
| 229796 | Bxb1-GT_TRE-mScarlet | Bxb1-GT donor plasmid for doxycycline-inducible mScarlet expression |
| 229797 | Bxb1-GA_CAG-rtTA-T2A-mTagBFP2 | Bxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2 |
| 229798 | Bxb1-GA_TRE-jRCaMP1b | Bxb1-GA donor plasmid for doxycycline-inducible expression of jRCaMP1b calcium reporter. |
| 229799 | Bxb1-GA_CAG-EGFP | Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of EGFP |
| 229800 | Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent | Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a |
| 229801 | Bxb1-GT_CAG-mRuby2 | Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2 |
| 229802 | Bxb1-GA_CAG-mStayGold | Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold |
| 232746 | pIVT-Bxb1 | IVT template for making modRNA encoding Bxb1 |
| 232747 | pIVT-evoBxb1 | IVT template for making modRNA encoding evoBxb1 |
| 232748 | pIVT-eeBxb1 | IVT template for making modRNA encoding eeBxb1 |
| 232749 | pIVT-Bxb1-I87L | IVT template for making modRNA encoding mutant Bxb1 modRNA (I87L) |
| 232750 | pIVT-Bxb1-I87L, A369P, E434G | IVT template for making modRNA encoding mutant Bxb1modRNA (I87L, A369P, E434G) |
| 232751 | pIVT-p53DD | IVT template for making modRNA encoding p53DD, a mutant p53 that lacks DNA binding ability |
| 232752 | pIVT-Flp-2A-BleoR | IVT template for making bicistronic modRNA encoding Flippase and Zeocin resistance gene |
| 232753 | pIVT-PuroR | IVT template for making modRNA encoding the Puromycin resistance gene |
| 232768 | pIVT-mGL | IVT template for making modRNA encoding mGreenLantern |
| 232773 | pIVT-iCre | IVT template for making modRNA encoding iCre |
| 241373 | Bxb1-GT-CAG Donor | Bxb1-GT donor plasmid with CAG promoter, WPRE and polyA signal for constitutive transgene expression (empty) |
| 241374 | Bxb1-GA-CAG Donor | Bxb1-GA donor plasmid with CAG promoter, WPRE and polyA signal for constitutive transgene expression (empty) |
| 241375 | Bxb1-GA-Donor_UbC | Bxb1-GA donor plasmid for cloning of payloads <20 kb with UbC promoter for expressing landing pad enrichment marker |
| 241377 | Bxb1-GA-hEF1a-mStayGold | Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with hEF1a promoter |
| 241378 | Bxb1-GA-UbC-mStayGold | Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with UbC promoter |
| 241379 | Bxb1-GA-CBh-mStayGold | Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CBh promoter |
| 241380 | Bxb1-GA-Bactin-mStayGold | Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with B-actin promoter |
| 241381 | Bxb1-GA-RSV-mStayGold | Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with RSV promoter |
| 241382 | Bxb1-GA-SV40-mStayGold | Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with SV40 promoter |
| 241383 | Bxb1-GA-CMV-mStayGold | Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CMV promoter |
| 241384 | Bxb1-GA-PGK-mStayGold | Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with PGK promoter |
| 241385 | Bxb1-GA-ShortCAG-mStayGold | Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with ShortCAG promoter |
| 241386 | Bxb1-GA-CpG-free-mStayGold | Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CpG-free promoter |
| 241387 | Bxb1-GT-CAG-mStayGold | Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CAG promoter |
| 241388 | Bxb1-GT-hEF1a-mStayGold | Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with hEF1a promoter |
| 241389 | Bxb1-GT-UbC-mStayGold | Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with UbC promoter |
| 241390 | Bxb1-GT-CBh-mStayGold | Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CBh promoter |
| 241391 | Bxb1-GT-Bactin-mStayGold | Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with B-actin promoter |
| 241392 | Bxb1-GT-PGK-mStayGold | Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with PGK promoter |
| 241393 | Bxb1-GT-Dox-AIO-TetOn_ETV2_Down-Tandem | Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of ETV2 |
| 241394 | Bxb1-GA_AIO-GZV-SynZiFTR_ETV2_Down-Tandem | Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of ETV2 |
| 241395 | Bxb1-GA_AIO-TetOn_ISL1-LHX3_Down-Tandem | Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of ISL1 and LHX3 |