Genetic code expansion reveals site-specific lactylation in living cells reshapes protein functions.
Shao C, Tang S, Yu S, Liu C, Zhang Y, Wan T, He Z, Yuan Q, Wu S, Zhang H, Wan N, Zhan M, Tan RX, Hao H, Ye H, Wang N
Nat Commun. 2025 Jan 8;16(1):227. doi: 10.1038/s41467-024-55165-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232155 | pNEU-KlacRS | Site-specific incorporation of L-lactyl-lysine into target proteins in mammalian cells. |
| 232156 | pcDNA-mCherry-TAG-EGFP | A fluorescence reporter to show incorporation of target unnatural amino acids. |
| 232157 | pEvol-MmKlacRS1 | Site-specific incorporation of L-lactyl-lysine into target proteins in E. coli cells. |
| 232159 | pEvol-chKlacRS-WT | A chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells. |
| 232160 | pEvol-chKlacRS-IPYE | A chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells. |