Construction of a CRISPR-Cas9-Based Genetic Editing Tool for Serratia marcescens Using a Stationary Phase Promoter and Its Application in Putrescine Production.
Gou L, Liu D, Fan TP, Deng H, Cai Y
Biotechnol Bioeng. 2025 May;122(5):1233-1244. doi: 10.1002/bit.28949. Epub 2025 Feb 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232354 | pBBR1-P2-SpCas9 | The plasmid pBBR1-P2-SpCas9 employs pBBR1MCS-2 as the vector, with Cas9 protein derived from S. pyogenes and the stationary phase promoter P2 sourced from S. marcescens HBQA7. |
| 232355 | pEdit | The pEdit series plasmids are derived from the pTarget series plasmids by inserting homologous recombination arms targeting the gene of interest, which enables gene knockout or gene insertion at the t |