Engineered nucleocytosolic vehicles for loading of programmable editors.
Geilenkeuser J, Armbrust N, Steinmassl E, Du SW, Schmidt S, Binder EMH, Li Y, Warsing NW, Wendel SV, von der Linde F, Schiele EM, Niu X, Stroppel L, Berezin O, Santl TH, Orschmann T, Nelson K, Gruber C, Palczewska G, Menezes CR, Risaliti E, Engfer ZJ, Koleci N, Schmidts A, Geerlof A, Palczewski K, Westmeyer GG, Truong DJ
Cell. 2025 May 15;188(10):2637-2655.e31. doi: 10.1016/j.cell.2025.03.015. Epub 2025 Apr 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 232427 | pCMV_ENVLPE+ | Expression plasmid to produce ENVLPE+ particles |
| 232428 | pCMV_iPE-C_P2A_Csy4 | Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection |
| 232429 | pCMV_ABE8e–superNLS | Expression plasmid for the ABE8e base editor with superNLS |
| 232430 | pCMV_Cas9–superNLS | Expression plasmid for the SpCas9 nuclease with superNLS |
| 232431 | pCMV_miniENVLPE | Expression plasmid to produce miniENVLPE particles |
| 232432 | pU6_empty-sgRNA(PP7) | empty gRNA backbone which contains the PP7 aptamer in the scaffold tetraloop |
| 232433 | pU6_rd6_pegRNA_(PP7-C4-Q1) | pegRNA with optimized 3' modifications to correct the rd6 mutation |
| 232434 | pU6_rd6_nsgRNA(PP7) | nsgRNA for PE3b correction of the rd6 mutation, contains a PP7 in the scaffold tetraloop |
| 232435 | pU6_rd12_pegRNA_(PP7-C4-Q1) | pegRNA with optimized 3' modifications to correct the rd12 mutation |
| 232436 | pU6_rd12_nsgRNA(PP7) | nsgRNA for PE3b correction of the rd12 mutation, contains a PP7 in the scaffold tetraloop |
| 232437 | pU6_HEK3+1T>A_(PP7-C4-Q1) | pegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locus |
| 232438 | pU6_B2M_sgRNA(PP7) | sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop |
| 232439 | pCMV_CreNLS_PP7 | expression plasmid to produce Cre-NLS mRNA with a PP7 aptamer in the 3' UTR |
| 232440 | pCMV_mGL_PP7 | expression plasmid to produce mGreenLantern mRNA with a PP7 aptamer in the 3' UTR |
| 232444 | pU6_HEK3_nsgRNA(PP7) | nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop |