Jonathan Ploski Lab: Ploski Lab Plasmids 2025
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233040 | pAAV-EF1a-mHFE(H67D)-IRES-GFP-WPRE-pA | To express mouse homeostatic iron regulator (HFE)(H67D) from an EF1a promoter. It also encodes GFP after an IRES sequence |
| 233041 | pAAV-EF1a-DIO-FLAG-mouse Beta Adrenergic Receptor (#1) | To Express a CRE dependent Flag tagged Mouse Beta Adrenergic Receptor from a EF1a promoter |
| 233042 | pLenti-CMV-eGFP-hTau E14-WPRE | To Express a GFP human Tau E14 fusion protein from a CMV promoter |
| 233043 | pLenti-CMV-eGFP-hTau P301L-WPRE | To Express a GFP human Tau P301L fusion protein from a CMV promoter |
| 233044 | pLenti-CMV-eGFP-hTau AP- WPRE | To Express a GFP human Tau AP fusion protein from a CMV promoter |
| 233047 | pLenti EFS-GFP-pa (opposite orientation)- TRE3g-Flag-GluN2B-WPRE | To express GFP from an EFS promoter in the 3'-5' direction and Flag-GluN2B from a TRE3g promoter in the 5'-3' direction |
| 233048 | pAAV CMV-d2eGFP-pA | To express D2eGFP from a CMV promoter |
| 233052 | pAAV CMV-SEP-GluR2(Q)-pA | To express a SEP tagged GluR2(Q) from a CMV promoter |
| 233053 | pAAV-CMV-Flag-Rat Arc-Arc- Genomic 3'UTR | To express Flag-tagged Rat Arc from a CMV promoter. The Arc gene contains the Rat Arc native Genomic 3'UTR containing two introns |
| 233054 | pAAV-Tre3G-Flag-Rat Arc-Arc 3' UTR | To express Flag-tagged Rat Arc from a TRE3g promoter. The Arc gene contains the Rat Arc 3'UTR containing NO introns |
| 233055 | pAAV-Tre3G-Flag-Rat Arc-SV40 3' UTR | To express Flag-tagged Rat Arc from a TRE3g promoter. The Arc gene contains SV40 3' UTR |
| 233056 | pAAV-Rat SARE-Arc Min Promoter-Flag Arc-Arc Genomic 3'UTR | To express Flag-tagged Rat Arc from a Rat SARE-Arc minimal promoter. The Arc gene contains the Rat Arc native Genomic 3'UTR containing two introns |
| 233057 | pAAV-Rat SARE-Arc Min Promoter-Flag Arc-SV40 3'UTR | To express Flag-tagged Rat Arc from a Rat SARE-Arc minimal promoter. The Arc gene contains a SV40 3' UTR |
| 233058 | pAAV-Rat SARE-Arc Min Promoter-D2GFP-SV40 3'UTR | To express D2GFP from a Rat SARE-Arc minimal promoter. The Arc gene contains the Rat Arc native SV40 3'UTR containing two introns |
| 233059 | pLenti-0.5Syn-Exon 1 of BNDF-GFP-WPRE | To express GFP from a 0.5 bp synapsin promoter. The non-coding BDNF exon one is 5' to the BDNF start codon. |
| 233060 | pLenti-0.5Syn-D2GFP-WPRE | To express D2GFP from a 0.5 bp synapsin promoter. |
| 233061 | pLenti-0.5Syn-7 MInGFP-WPRE-pA | To express 7 Min GFP from a 0.5 bp synapsin promoter. The non-coding BDNF exon one is 5' to the BDNF start codon. |
| 233273 | pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #1) | To express GFP from a CMV promoter and to express an shRNA targeting Rat Nurr1 |
| 233274 | pAAV-CMV-GFP-pA U6 shRNA (Rat Nurr1 #5) | To express GFP from a CMV promoter and to express an shRNA targeting Rat Nurr1 |
| 233275 | pDsRed1-CMV-Rat Nurr1-DsRed1-pA | To Express a Rat Nurr1-DsRed fusion protein from a CMV promoter |