Sensitive dissection of a genomic regulatory landscape using bulk and targeted single-cell activation.

Vucicevic D, Hsu CW, Lopez Zepeda LS, Burkert M, Hirsekorn A, Bilic I, Kastelic N, Landthaler M, Lacadie SA, Ohler U
Cell Genom. 2025 Oct 8;5(10):100984. doi: 10.1016/j.xgen.2025.100984. Epub 2025 Sep 9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
233066	pPB-CAG-dCas9-VPR-pgk-hph	Piggybac CRISPRa construct
233067	pPB-CAG-KRAB-dCas9-P2A-mCherry-pgk-hph	Piggybac CRISPRi construct