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A lentiviral fluorescent reporter to study circadian rhythms in single cells

Gabriel CH, Lehmann L, Ahlburg J, Achim Kramer A
bioRxiv 2025.06.30.661926 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
240105pL6puro-pmRevErbA-Venus-NLS-PestA lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein)
240106pL6puro-JeT-pmRevErbA-Venus-NLS-PestA lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.
240107pL6puro-JeT-pmRevErbA-mScaI3-NLS-PestA lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.
240109pL6puro-JeT-pmRevErbA-mClover3-NLS-PestA lentiviral (yellow-green) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.
240111pL6puro-JeT-pmRevErbA-Electra2-NLS-PestA lentiviral (blue) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.
240112pL6puro-pSV40(1)-pmRevErbA-Venus-NLS-PestA lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.
240113pL6puro-pSV40(2)-pmRevErbA-Venus-NLS-PestA lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter.
240114pL6puro-pSV40(3)-pmRevErbA-Venus-NLS-PestA lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.
240115pL6puro-pSV40(4)-pmRevErbA-Venus-NLS-PestA lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter.
240116pL6puro-pSV40(1)-pmRevErbA-mScaI3-NLS-PestA lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.
240117pL6puro-pSV40(3)-pmRevErbA-mScaI3-NLS-PestA lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.
240118pL6puro-pSV40(4)-pmRevErbA-mScaI3-NLS-PestA lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter.

Antibodies from Article