RECKLEEN: a lambda Red/CRISPR-Cas9 based single plasmid platform for fast, efficient, markerless, and scarless genome editing in Klebsiella pneumoniae
Elsayed E, Stukenberg D, Schmeck B, Becker A
Research Square (2025) [Preprint]. doi: https://doi.org/10.21203/rs.3.rs-5859838/v1.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233456 | Reckleen_1_Cas9 | Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae |
| 233457 | Reckleen_2_SpGCas9 | Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae |
| 233458 | Reckleen_3_multieditdropout | Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae |
| 233459 | Reckleen_3plus_sgRNA_ptet_pos4 | Level 1 part, Ptet sfgfp dropout sgRNA for position 4, for building RECKLEEN plasmid with multiple sgRNAs |
| 233460 | Reckleen_3plus_sgRNA_ptet_pos5 | Level 1 part, Ptet sfgfp dropout sgRNA for position 5, for building RECKLEEN plasmid with multiple sgRNAs |
| 233461 | Reckleen_3plus_sgRNA_ptet_pos5a | Level 1 part, Ptet sfgfp dropout sgRNA for position 5a, for building RECKLEEN plasmid with multiple sgRNAs |
| 233462 | Reckleen_3plus_sgRNA_ptet_pos5a1 | Level 1 part, Ptet sfgfp dropout sgRNA for position 5a1, for building RECKLEEN plasmid with multiple sgRNAs |
| 233463 | Reckleen_3plus_sgRNA_ptet_pos5a2 | Level 1 part, Ptet sfgfp dropout sgRNA for position 5a2, for building RECKLEEN plasmid with multiple sgRNAs |
| 233464 | Reckleen_3plus_sgRNA_ptet_pos5b | Level 1 part, Ptet sfgfp dropout sgRNA for position 5b, for building RECKLEEN plasmid with multiple sgRNAs |
| 233465 | Reckleen_3plus_sgRNA_ptet_pos5b1 | Level 1 part, Ptet sfgfp dropout sgRNA for position 5b1, for building RECKLEEN plasmid with multiple sgRNAs |
| 233466 | Reckleen_3plus_sgRNA_ptet_pos5b2 | Level 1 part, Ptet sfgfp dropout sgRNA for position 5b2, for building RECKLEEN plasmid with multiple sgRNAs |